Why am I not getting pellet after centrifugation of gold nanoparticle synthesized by seed method(CTAB)?

31 March 2023 1 8K Report

We centrifuged twice at 12000 and 14000 rpm but still no pellet was found.

What size did you expect? The smaller size of gold NP needs more rpm. A little amount of salt helps the precipitation of gold NP. However, you should be very careful with adding salt to the gold solution. It easily aggregates god NPs. If you provide pictures of your products before and after of the centrifuge, it will help us to find a better solution

Badges
Science topic

More Eshaa Umashankar's questions See All

Can I combine Survey Data and Actual project Revenue Data (Primary data) in regression analysis?

Dear RG Members, I am doing a study at a team-level from a software services organization collecting data by surveying the team lead and the members. My independent variables (IVs) are...

27 August 2021 9,347 3 View

Need weather and metrological data for forecasting or predicting the agriculture in Saudi Arabia?

Need weather and metrological data for forecasting or predicting agriculture in Saudi Arabia? This is for the purpose of one of the project by my fellow researchers. Kindly share the same.

11 August 2020 8,384 4 View

What are the high impact journals available in sustainability and sustainable development domain?

24 June 2020 4,152 11 View

What are the key topics or sub topics available in sustainability or sustainable development as per UN SDG?

What are the key topics or sub topics available in sustainability or sustainable development?

24 June 2020 1,020 11 View

What are the COVID-19 papers retracted from journals?is it clinical, treatment, diagnosis or AI based prediction? Why?

What are the COVID-19 papers retracted from journals? is it related to clinical, treatment, diagnosis or AI-based prediction? Why

07 June 2020 7,495 3 View

Where can we get the scanned X-ray or CT Scan images of COVID-19 virus patients ? Any repository available?

28 May 2020 7,464 4 View

Which is giving accurate results in COVID-19 diagnosis? whether X-ray images or Scan? Any relevant links or research?

24 May 2020 2,376 6 View

Can any one please let me know the informations regarding metal matrix composite of AL 6061+ ZIRCONIUM+ FLY ASH?

i am working on the project of study of machinability of Alluminium 6061, zirconium, fly ash composites. before i take a step ahead i want to get some details about how the results of this...

25 July 2016 9,886 2 View

What type of Procurement strategy to be followed for constructing a ski-resort and a rehabilitation center?

Which would help the client and the contractor in a long run.

19 April 2016 2,794 5 View

How to Minimise the waste generation in Construction Projects by design?

Where design plays a major role in earlier stage of construction. So by proper design can we really reduce the waste generation(minimum)

22 March 2016 8,568 4 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Hello researchers Is this a random laser or just fluorescence?

I am using Rhodamine6G as gain medium and silver nanoparticles as scatterers on a microscope slide and laser input 532 nm comes from above.

09 August 2024 9,894 2 View

How to prepare the nanoparticle treated fungal sample for Environmental SEM analysis?

A fungal strain was treated with nanoparticles. We want to do an environmental SEM analysis. So could anyone share your views on preparing the sample? Thank you.

07 August 2024 5,307 1 View

How does grain and grain boundary affect the ceramic when studying its dielectric properties?

I am not able to get good literature and the physics behind how first these grains and grain boundaries arises out of no where when we make a pellet to study its dielectric properties and then how...

07 August 2024 5,177 3 View

Binder for ZnO nanoparticles in graphite paste electrode?

I have been using paraffin, but the deposited ZnO still detach from electrode. What is the best binder to modify graphite paste electrode with ZnO nanoparticles?

03 August 2024 4,624 3 View

Can i quantify a nanoparticle encapsulated in cell culture medium by hplc?

Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...

03 August 2024 2,730 1 View

What is the most effective way to sterilize camelina seeds?

I'm working on camelina seed sterilization and have some doubts about the process. I followed a protocol used by my lab mate, but I'm unsure about a couple of things: Is it safe to use 10% bleach...

02 August 2024 1,178 3 View

PBMC infection with virus?

In my study, I intend to infect PBMCs with SARS-CoV-2. After that, I will analyze NK cells by flow cytometry to see if their phenotype changes or if they show degranulation. After the infection, I...

01 August 2024 4,403 4 View

How to increase protein lysate concentration?

I am trying to prepare samples for IP. The pellet size is good and I am using 1% Tx-100 and using a syringe to further lyse the cells and maintain protein-protein interactions. I keep having a...

01 August 2024 7,199 1 View

What is the best practice for resuspending cell pellets during competent cell preparation (both electro and comp)?

I'm fairly new to making homemade comp cells and have done so with some mild success. What is generally an acceptable way of resuspending comp cells during wash steps? I've been told to swirl the...

31 July 2024 8,309 0 View