I am working with ATM plasmids of bacterial origin.

The workflow is: PCR → digestion with DpnI → transformation using DH5a cells

I followed every step of the protocol, the gel I ran showed the desired DNA band, but nothing appeared on the LB agar plate. I don't know what went wrong and I am very frustrated. Could someone advise me on how to improve? That would mean a lot to me.

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