Why am I facing problem in transformation of pKD46 plasmid in Pasteurella multocida?

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Michael J. Benedik

Are you growing at 30oC or less? pKD46 is temperature sensitive for replication.

Junaid Ali Meeran

Dear Michael J. Benedik

I have grown them at 30 C, and I have also used Top10 as control during my transformation. Controls are working good but pKD46 is not being transformed in Pasteurella multocida.