Why absolute ethanol react with total antioxidant capacity reagent and gives intense dark blue colour when it used as a blank.?

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Andrei Blasko

The impurities from absolute ethanol like propanol, iso-butanol, n-butanol, isopropanol can react with your reagent.

Faiq H S Hussain

Try to distillate ethanol carefully to remove the impurities ( if any ).

Another reason may be formation of coordination bonds, is it possible to use non-polar solvent and free from O and N?

Good Luck

Ramandeep Kaur Mankoo

due to impurites present in ethanol. distill ethanol 2-3 times before using it as blank.