I'm working now at a test for visible agglutination of PCR (C-reactive protein) from human serum on a slide. I used the goat anti-human antibody to coat the carboxylate latex particles with 1 micros. I used EDC for coupling with MES buffer. During the incubation with IgG I took the samples (after 2h at romm temperature) and the aglutination was visible for positive control and also for negative control. After coupling I put the latex in other buffer for wash and quench based by Tris and glycine but with a high pH=9. This produce an inactivation of agglutination because it has not appeared. What happened ?
Thank you very much.
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