it is well-known that the 18s rRNA lacks Poly-A tail. I was wondering whether, why 18s rRNA gene can be amplified when cDNA is synthesized by oligo-dT?
See when total RNA is used as template its rRNA are most stable RNA type, followed by mRNa and tRNA, however our templates are never ideal they have broken rRNA parts, which intern prime for rRNA amplification. Try doing a ribosomal clean up and then do oligoDT CDNA synthesis.. your result would be more enriched