I have problems with the data I get from the soil samples with diesel. Anyone who is working or have worked with this??
Thankyou!!!
I'm looking to try this in place of normal goat serum for a wisteria floribunda lectin stain. In my IF protocol, 10% of the final volume of my blocking solution is goat serum. If I were to go the...
07 February 2021 7,520 6 View
I'm working with 40um fresh frozen (non-perfused) sections of rat brain tissue and I'm staining for wisteria lection (immunofluorescence). After staining, my tissue has high background and a weak...
10 November 2020 3,620 7 View
Good morning, dear community, I have a preliminary report from an in silico analysis of non-coding short sequences against the SARS-CoV-2 virus. I'd like some advice about which indexed journals...
18 July 2020 5,365 1 View
Hi !! I've seen a lot of arguments in favour and against the use of a mask while running outdoors. I think it's just that some people are not comfortable enoughwearing one, but maybe there is some...
08 July 2020 4,927 2 View
I'm trying to edit an existing citation style to suit my needs (Journal of Tropical Ecology). I edited it so the inline citations go from newest to oldest reference (Smith et al 2019, Jones et al...
15 June 2020 9,926 4 View
Hello, I am trying to look at the efficiency at which a Gene is cut after tamoximen treatment. Thus we got a set of primers that can tell us weather the Gene is cut or not. We wanted to measure...
02 February 2020 7,599 3 View
As you can see, most of our colonies express GFP while we expected to get only 50% of these or less.
04 December 2017 8,378 3 View
I am getting some extra bumps in my qrtpcr for actin. I see it in the nyc and in the x10*5 standard. So I think is primer dimer forming when there is not enough template. I tried adding more cDNA...
05 November 2017 5,899 7 View
I ran a 15ug western blot using protein from treated mammalian cells. The top bands are Ligase 4 and the bottom bands are B-Actin. I am not sure why there is smearing in the Ligase 4 bands but not...
12 September 2017 3,730 2 View
Does Intrinsic vs. Extrinsic Motivation Play a Role in People who Exercise Alone or With a Partner? The independent variable for this experiment is whether they exercise with a partner or not. The...
21 May 2016 8,266 3 View
Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...
03 March 2021 8,796 3 View
Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...
03 March 2021 5,999 3 View
I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance
03 March 2021 3,568 1 View
Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...
03 March 2021 5,978 5 View
What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...
03 March 2021 1,401 3 View
I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...
03 March 2021 8,920 3 View
dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...
03 March 2021 6,994 5 View
I'm involved in a study of odor control technologies for municipal wastewater treatment plant. One of the control options involves a chemical 2-stage (acid/alkaline) packed bed scrubber. The...
03 March 2021 3,661 2 View
1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature-based reflection)? Thank you for your time and for...
03 March 2021 2,870 3 View
I'm dealing with a mediation model and am using the PROCESS module in SPSS. Due to SPSS and PROCESS being limited in the imputation methods - being unable to handle multiple imputation - the other...
02 March 2021 4,362 1 View