For Mechanosensitive ion channel, I would like to compare their membrane tension. Most of their values can be found, but this one is not available.
Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...
06 August 2024 1,495 2 View
Hi all, may I ask you for advice about treating monolayer culture cell line with essential oil. Due to the hydrophobic character of the oil, I am concerning how can it equally affect to all the...
31 July 2024 5,301 2 View
I'm very interested in biotechnology and medical research and I'd like to meet a professional here and talk about it together
28 July 2024 3,798 4 View
Where do I download the codes, databases commonly used by researchers?
16 July 2024 295 0 View
Hello, I was attempting to download some sequences data from NCBI. The format "gene features" could help me extract certain gene fractions using the names, but I found that it seems to be...
04 July 2024 9,875 1 View
"Nuclear-mitochondrial DNA segments (NUMTs) are mitochondrial DNA (mtDNA) fragments that have been inserted into the nuclear genome." (Xue et al., 2023) I would like to know under this...
20 June 2024 603 1 View
Can Opti-MEM without serum be used for transfection? Myotubes are typically at a high density upon successful differentiation. Does this reduce the efficiency of transfection?
15 May 2024 5,843 0 View
If the depolarization ratio of a Raman peak is less than 0.75, it is a polarized band, and if it is greater than 0.75, it is a depolarized band.
04 May 2024 2,829 0 View
After treating my membrane with the primary antibody, the destaining buffer was accidentally used instead of tbst buffer. Immediately after use (about 3 seconds later) it was replaced with tbst...
24 April 2024 2,625 1 View
Hi I have a few oligonucleotides with G4 forming sequences that I have checked they form G4 by CD spectroscopy. I plan to double check G4 formation by using Thioflavin T (ThT) fluorescence...
27 March 2024 4,257 0 View
I'm currently working on calculating the collision cross section (CCS) for various ions, and I'm facing challenges when dealing with sodiated and multiply charged ions. Most of the resources I’ve...
08 August 2024 8,329 0 View
I am trying to coat my micelles and PLGA based nanoparticles with cell membrane using an Avanti mini extruder according to literature . I use 200nm membrane but my particles end up to be 400nm And...
30 July 2024 3,294 3 View
I'm having problems with ion exchange chromatography. After applying my sample (a fluorescent protein), a preferential path inevitably forms. The matrix I use is DEAE-Sephacel. Could someone...
27 July 2024 6,605 0 View
Does it exist some special technique for cutting ceramic membranes for Cross-sectional FESEM or SEM images?
25 July 2024 5,482 2 View
Hello, I am currently working with Malaria Pf/Pan nitrocellulose membranes coated for malaria detection. After drying the coated membranes in an Incubator (overnight at 37°C), I've noticed that...
16 July 2024 7,937 1 View
I have made an unpleasant discovery that I seemed to use these terms incorrectly. 'Alluvial' seems to mean "out of the river channel", flood-plain' etc. While all these cross-bedded...
15 July 2024 2,073 3 View
we have a nafion 117 membrane and we want to know if it has the properties claimed by the sellers. what are the tests available ?
13 July 2024 503 1 View
The column is used in Ion Exchange Chromatography of Monoclonal antibodies and the method of analysis is based on pH gradint.
13 July 2024 7,462 1 View
NA
11 July 2024 8,969 1 View
Would an average spot welder suffice for welding tabs for pouch cell electrodes instead of an ultrasonic one? Why would an ultrasonic welder be better than a regular spot welder?
10 July 2024 8,763 0 View