Is it suitable to stabilise human blood cells by RNA-Later to perform DNA expression studies?

Human blood cells stabilised by RNA-Later to perform DNA expression studies. It is a good way to store white blood cells to perform subsequently the isolation of RNA? Or is it preferable to store...

23 June 2015 3,340 3 View

Can someone give me a suggestion on the cloning procedures?

11 March 2015 1,728 4 View

Does anyone have experience with, or literature on, bone plow or rake phenomenon using locking plate fixation ?

When bone is cut by locking screws. In my experience in metaphyseal bone.

08 February 2015 5,366 2 View

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I have a list of genes annotated with InterProscan. I would like to summarize the results plotting the GO category and look at which major function or biological processes those genes are involved...

12 August 2014 455 2 View

Could somebody please give me the nucleotide sequence of the TEV protease recognition site?

I would like to use this sequence for TAP of protein complexes.

17 February 2014 7,498 7 View

Which activities should be the most important for academic people?

Normally an academic dedicates do some hours per week to research, some to teach and some to administration of the Department or Faculty. Some hours are also dedicated to external work, as...

01 November 2013 4,783 3 View

Can anyone advise on antibodies against "histone locus bodies" that work in imunofluorescence?

I am trying to identify histone locus bodies in a prostate cancer cell line (PC-3)

09 July 2013 280 3 View

Hek-293 stable cell line problems?

I am having a problem with the selection of my HEK-293 cells. I started the selection with G418 two months ago and I still have in my flask untransfected cells that don't die. What can I do? Try...

20 June 2013 1,849 19 View

What are the best markers for the identification of Neutrophils, eosinophils and circulating dendritic cells in whole blood by flow cytometry?

I need to study these cells in whole blood samples. I normally use the protocol "Lyse-no-wash". It could be used to study these cells, too?

05 April 2013 5,771 10 View

Are the telomere length different in human skeletal muscle upper limbs respect lower limbs?

Is there any evidence that telomere length in human skeletal muscles is limb specific?

03 April 2013 8,558 1 View

Why should we choose deep learning to machine learning? Why is CNN better for image classification? Can I get Typical Answer?

What Characteristics makes CNN work better?

03 March 2021 1,458 4 View

What are some of the research gaps in machine learning and artificial intelligence in africa?

i would to know some of the research gaps in the artificial intelligence field in most african countries.

03 March 2021 6,145 3 View

Anyone plz suggest relevant topic for research in image processing+Deep learning.?

I have selected brain tumor images ...but now found that already lots of research done n this topic.

03 March 2021 5,774 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Can you share me how teacher education is managed in your respective national educational system?

I feel that the practice in teacher education in my country is below the expected performance level due to very poor management system. Hope I will learn something from your experiences.

02 March 2021 1,516 4 View

Can NFL theorem be valid in infinite search space in ML?

NFL theorem is valid for algorithms training in fixed training set. However, the general characteristic of algorithms in expanded or open dataset has not been proved yet. Could you show your...

01 March 2021 1,189 3 View

What is the difference between L1 and L2 regularization in machine learning?

L1 and L2 regularization

28 February 2021 4,187 3 View

How to eliminate Steady state error from Reinforcement learning genetic algorithm code?

The following code (see 1st 2 images attached) is used to produce PID controller values that are designed to control the system (G). The code finds the PID controller values (noted as k) by using...

28 February 2021 6,560 14 View

Is there any Machine Learning/AI algorithm that lets you predict with incomplete features without missing value imputation process?

For instance, the model is trained with A,B,C,D,E features to predict F target. Then, I want to make prediction on a new data, but with only A,C,D,E is known without doing missing value imputation...

28 February 2021 1,556 6 View

How can I represent SDS-PAGE results for machine learning analysis?

I am trying to classify and analyze the results of an SDS-PAGE based array for bacterial detection using machine learning, but I have trouble finding the best way to represent the results with...

27 February 2021 9,176 3 View