I want to determination the root activity of maize, who knows a fast way to determination the root activity? Thank you for your kind help and comments?
You may look at this article. https://www.jircas.go.jp/sites/default/files/publication/jarq/05-3-001-006_0.pdf
There is no fast way since you need to prepare the samples then measure absorbances :)
Grow in water solution and use a video.Regards
Introduce a hydroponic and grow maize. it will be easy for observation
The root activity was estimated following the TTC method (Comas
et al., 2000), and root activity was expressed as the
capacity of root deoxidization (mg g−1 h−1). In short, five hundred
milligrams (500 mg) of root sample were dipped in equal amounts (0.4%)
of TTC and phosphate buffer mixed solution (10 mL) followed by placing
in the dark for 1–3 h at 37 °C. After that, to stop the reaction of the
solution, we added 2 mL of 1 mol L−1 H2SO4. Then, the dipped roots
were blended, followed by transfer into a new tube containing ethyl
acetate cleaning solution (10 mL). The absorbance
Incompletely cross-linked pdms are difficult to remove.
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