I have some studies on in stu hybridization in cryosection or paraffin section but not in cultured cells. I have searched and find some protocols but I could not imagine how to do. It is not clear for me. Especially cultivation step before and after is described like first seeding of cells on poly-L-lysine coated microscopic slides and next incubation. At this point I think some petri dishes should be used for keeping slides in sterile condition and handle it easly. It seems complicated. I hope any body has such kind of experience and give me some advice. Thank you.
Growing and maintaining cells in culture slides is not as complicated as you might think as you can get microscope slides that are made specifically for this kind of work, however they can be costly. The link below is just one example, I'd encourage you to shop around and ask the company rep if they can send you out some sample slides for free.
http://www.sigmaaldrich.com/catalog/product/sigma/c7182?lang=en®ion=NZ
Using a petri dish or some other tray to help with handling does help, but isn't necessary for keeping them sterile as they will come with lids. You can get pre-coated poly-d-lysine slides or coat them yourself.
I had seen this kind of slide system but I couldn't imagine that is for in situ and I forgot it. Actually it is quite high price. I also do coating slides but I like the chamber. Thank you very much.
You could maybe coat the slide, then run the culture in a petri dish if you can't get any chamber slides, would be worth giving it a try. Sigma usually has high prices for things. Ask around different companies to see if someone will give you a discount or send you a free sample.
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