I would like to transform them with the same gene but in two different configurations. One with the gene of interest under Ptrc promoter and the other under Pt7 promoter.
maybe you can contact the groups of Prof. Kenneth Timmis (Braunschweig, Germany), Prof. Juan Luis Ramos Martín or Prof. Victor de Lorenzo (both Spain) which are experts in the field of Pseudomonas putida research. They should have a large KT2440 mutant and plasmid collection.