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I'm trying to express a gene in P.p. with pGAPZalpha B. I though to linearize with BglII, which is at the begining of pGAP, but I have other BglII restriction sites in my gene of interest. So, I...
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I would like to transform them with the same gene but in two different configurations. One with the gene of interest under Ptrc promoter and the other under Pt7 promoter.
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