Aloe tororoana is an endemic aloe species that grows exceptionally on the rock cliffs of Tororo rock. We have been comparing its phytochemical composition with those aloes found growing on Mt. Elgon at almost the same altitude. My keen interest is to determine the content of anthraquinone glycosides in A. tororoana, preferably using HPTLC.
Dear
there are two methods using HPLC-UV:
These two methods are developped in a published paper by Derksen et al.(2004) in
Phytochem Anal. 15(6):397-406.
Best regards
Dear Researcher
Hope this paper will help you in solviving your problem.
Dear Moses, You can use HPLC/UV-VIS Analysis. Two related papers are attched that may be helpful.
http://webap.cmu.edu.tw/wr/project/AA-CC00_20120509112208.pdf
Thank you Yogesh, Dr. Shanti, Alessandro, and Barnaba for your answers and links you have sent me.
The brief steps are:
First of all you isolate the pure fraction from plant extracts using following techniques. First run TLC to know the type of fractions. After that Column Chromatography with HPLC, after isolated pure fraction run FT-IR, LC-MS, HNMR, CNMR for structure elucidation. You can read my papers too.
Dear Moses
Please find some paper related to your query, which definitely helps you for designing your experiments.
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