How much cell pressure and back pressure should I apply to start for triaxial test for different types of soil?

We have recently purchased a triaxial test instrument (MATEST) for testing different types of soil. After installing software, we are facing a issue of saturation where the samples does not...

23 February 2021 7,194 4 View

How to produce huge amount of protoplasts from Fusarium graminearum?

Hi, all I am using some protocol for protoplast production from Fusarium graminearum (Fg), but I need them in a huge amount. Sometimes the protocol worked well but most of the time failed....

14 February 2021 2,286 4 View

Whom should be given the priority for vaccination for Covid-19?

Mass vaccination for Covid-19 is going to start very soon. Whom do you think should be given priority for vaccination? Senior citizens, frontline workers or students? Why?

24 December 2020 8,267 6 View

How to do Fusarium graminearum protoplast transformation using desired plasmid?

I would like to specify my question regarding the transformation of plasmid DNA into F graminearum protoplast. I am using the following reaction mixture (30%PEG 50ul, 200ul protoplast, and 20ug of...

14 December 2020 4,251 1 View

How could we redope Ni deposited PPy?

We prapare Ni deposited polypyrrol (PPy). But after Ni deposition, the conductive polymer is depoded and in CV analysis, the observed current value is very low. An advice is expected from the...

02 December 2020 9,204 3 View

Are polymer viscosity, storage and loss moduli volume-dependent?

It is welcomed to introduce any published paper in this respect.

26 November 2020 3,679 3 View

Could you explain the following situations, please?

I am trying to simulate a flexible antenna on ANSYS HFSS. Here, I have used "PDMS" as a substrate and "pec" as a probe (inner conductor) of a coaxial feeding. But during simulation, it is showing...

24 November 2020 800 5 View

What technique can be used to monitor carbon dioxide in natural waters?

I am looking for a method or a sensor for the monitoring of the dissolved carbon dioxide in wastewater and natural water.

22 November 2020 3,439 4 View

Can I get an explanation for the following reason, please?

My plan is to fabricate a conformal microstrip patch antenna. I am going through the simulation works first. I already simulated the planar antenna. Now I am trying to simulate it with different...

19 November 2020 2,961 2 View

I want to determine intracellular calcium concentration of Hela cells using Fluo-8 AM? Is there any protocol?

I also want to use Spectro fluorometer to measure calcium concentration. One more thing, after treating cells with calcium dye, Should I lyse the cells or not to measure calcium concentration?...

11 November 2020 7,757 3 View

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23 February 2021 4,014 3 View

Is ANSYS Fluent good for simulation of a three phase reactor(stusy of its hydrodynamics or mass transfer...)?

Hello everybody, I would like to simulate a three phase reactor and study its hydrodynamics. I would like to know which type of software is better? I have a good command of ANSYS Fluent and I am...

10 February 2021 4,303 3 View

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When producing a recombinant protein, harvested E. coli cells are normally refrigerated for 12-24 hours before homogenization to ensure protein inactivation due to foaming does not occur....

16 October 2020 7,373 0 View

How do you extract DNA from buccal swabs submerged in 70% ethanol using Qiagen DNeasy Blood and Tissue Kit?

Hi, I collected buccal swabs (cotton and foam) from nestlings and adult birds and stored the swabs in 70% ethanol. There are few different protocols on the web but I am wondering if anyone has a...

11 October 2020 6,103 3 View

What does over foaming correspond to in E.coli fed-batch fermentation?! How does foaming relate to cells metabolic status?

I'm currently experiencing overfoaming in my E.coli culture. My cells are used to produce a recombinant protein. The basic cell strain is BL-21 DE3. After changing my carbon source brand i am...

06 October 2020 3,946 3 View

What is the influence of the mold release agent on sound absorption properties of polyurethane foam ?

I'm an intern in a big company which makes flexible polyurehane foam for the automotive sector and I'm working on sound absorption properties of PU foam. I'm not chemist so I'm trying to improve...

23 September 2020 3,765 4 View

For construction of graphene based electrodes, what can be used as a substrate in lieu of nickel foam?

I am doing my thesis on the use of graphene for supercapcitor electrodes. Most examples I have read about use nickel foam as a substrate for electrode production. I will most likely be looking at...

16 August 2020 1,388 4 View

How to make safe electrical connection between cable and nickel foam for electrode?

Hello everyone, Currently, I use Ni foam as working electrode. To connect with potentiostat, I connect the Ni foam with copper wire and soldering in the joint. To prevent any contact between...

09 August 2020 9,250 2 View

Can I get a research topic on trending research area on automobile lubricant?

The alternative additives ( anti oxidation, Visco index enhancers, anti rusting, anti foaming etc ) to the conventional ones possibly locally sorted.

29 March 2020 5,893 3 View

How to get cel opening in a frothed PU foam?

I am making frothed PU foams (compressed air is mixed in). The foam only rises 1 to 3 mm during heating and the bubble struts of the foam tend to be strong, so they are difficult to open up. Does...

13 March 2020 2,212 4 View