Which simple method can be used to monitor levels of intracellular cAMP by using a non-confocal microscope equipped with UV lamp and filter sets?

I want to measure intracellular cAMP in various cells. Which simple method is available? I read some papers about EPAC BRET, but I am looking for a SIMPLE method. I have a decent microscope with...

09 October 2016 8,288 11 View

How can I measure the DNA alkyl products of cytotoxic agents such as ethylnitosourea?

I need to get information how I can measurre the alkyl products of ethylnitrosourea (e.g. O(6)-alkylguanine) in various tissues of mice fetuses (liver, lung, brain). I want to measure the...

09 October 2015 6,852 1 View

Does it make any sense to handle / culture human-hamster hybrid cells at room temperature?

In an experiment researchers have used human-hamster hybrid (AL) cells which contained a normal set of hamster chromosomes and one human chromosome 11. Several experiments with the cells were...

01 February 2015 6,546 2 View

Do I have to run a one-tailed Fisher's exact test in a replication experiment where the results are in support of the previously published data?

The problem is as follows: we have tried to replicate an experiment which has shown some positive effects of a certain treatment. My personal expectation was that the results were not...

10 November 2014 9,255 9 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

No title

01 March 2021 3,905 5 View

Is there any teachers based in UK who would like to take part in my study?

24 February 2021 4,833 3 View

Anyone give me assistance with analysing Gait Data?

I currently have collected Gyroscope/Accelerometer data (X/Y/Z) for my dissertation, but not very knowledgeable on how to process my data and apply Machine Learning? Can anybody help?!

24 February 2021 9,610 3 View

Inpatient DBT to reduce parasuicidal behaviour?

Currently trying to obtain as many pieces of research as possible for my dissertation around the implementation of DBT within the inpatient unit for those with a diagnosis of BPD. Primarily...

23 February 2021 5,654 2 View

What type of SPSS analyses should I use for my Dissertation?

I'm currently working on my Dissertation and I'm trying to think of the best analyses that I should use for my study. I'm using three questionnaires for this and two different groups, 1st group...

22 February 2021 1,351 4 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View

How do I check the quality of my viral RNA prior to metagenomic sequencing?

I am doing metagenomic sequencing without enrichment for an RNA virus. After my RNA is extracted from the blood I am doing library prep with the NEBNext Ultra directional RNA library prep kit for...

16 February 2021 5,101 2 View

Dissertation Topic Ideas for MSc Business Management?

Hello, I hope everyone reading this is doing well during these tough times. I am doing my masters in business management and considering dissertation topics. I'm interested in taking an...

15 February 2021 594 26 View

How to remove the species data from rda data frame in R (only remain the sites/samples point)?

Dear everyone, When I using the rda function in r, I met this question. As you can see in the figure, there are a lot of deep blue points which refers to the species. But I would like remain...

15 February 2021 8,982 2 View