Which will be best solvent to dissolve melatonin for use in cell culture?

06 June 2018 1 5K Report

Dear Colleague (s)

I am having little confusion regarding the best solvent to dissolve melatonin. Among ethanol, isopropanol and water, which will be best keeping in mind the duration of its half-life? I am finding it confusing in Sigma-Aldrich data sheet (Catalogue number # M5250).

William M Connelly

Melatonin isn't very soluble in water. I would dissolve it in DMSO, where it's soluble in the 10s of mM range, and then add the DMSO/melatonin mixture to your medium to get the appropriate melatonin concentration.

But if ethanol. iso and water are your only options, then go with ethanol. Just make sure you use a vehicle contrpl.

Badges
Science topic

Similar topics
Chemistry
Chemicals
Solvents

More Dayanidhi Jena's questions See All

What are the thumb rules for primer designing?

What are the thumb rules for primer designing and which websites I should rely upon for designing of my primers?

05 June 2018 2,239 3 View

How to do DEPC treatment of tips and tubes?

Can anyone suggest in detail or provide any link for DEPC treatment of pipette tips and eppendorf tubes?( As it is imperative to use DEPC treated tips and tubes for RNA extraction from samples).

02 March 2018 5,515 14 View

Injection of embryonic stem cells into a blastocyst stage can give rise to whole organism except placenta. Why it's possible in mice not in human?

Which factors potentiate mice blastocyst to expand into full organism (except extra embryonic tissues) whereas not human cells, upon injection of embryonic stem cells?

31 December 2017 8,093 4 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

Polymer composites suddenly solidifies?

I am making polymer composite by solvent mixing method. When I am trying to add filler solution in polymer solution it quickly get solidified. How to make them then uinform? It doesn't give time...

03 March 2021 7,540 4 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

How to polymerize TEOS?

I want to polymerize TEOS on a surface. I know that NH4OH is a preferred catalyst and an alcoholic solvent should be used .But I can not find certain amounts of each material to use. Does any one...

02 March 2021 1,404 6 View

Where can I find life cycle inventory or assessment of non woven materials?

Dear network, I am looking for the LCA or LCI or at least the energy balances of the non woven fabrics process for my master project, in specific for natural fibers, yet I have found...

02 March 2021 9,662 1 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Please suggest a good text book for "Research Methodology in Life Sciences". Which book would be good for PhD Scholars?

Please provide Book Title and author name

02 March 2021 9,059 3 View

How can extract ciprofloxacin hydrochloride disolve in water er to organic solvent such as dichloromethan or any other solvent?

I need a procedure to extract the ciprofloxacin hydrochloride from aqueous solution by using dichloromethane in detail,. I thank you very much.

01 March 2021 4,306 1 View

How to separate water from glycerol solvent in a solvent recovery vacuum distillation column?

I am currently doing my third year design project. I have been assigned to design a glycerol recovery column in vinyl acetate monomer production. The process unit before the recovery column is an...

01 March 2021 9,525 4 View

Which group formation theory do you recommend for projects that aim to bring together entrepreneurs for training for regional development?

I would like to have ideas about which theories of group formation are suitable for entrepreneurial training projects, with the aim of creating bonds, building trust, and collaboration for...

28 February 2021 4,223 2 View