i want to quantify saponin in Aloe vera genotypes using HPLC. Which steroidal saponin to be used as marker compound?? please suggest.
https://www.ncbi.nlm.nih.gov/pubmed/?term=aloe+saponin
hi,
u can use sitosterol, or stigmasterol as a reference compound.
Instead of sterols, you can use saponin from other sources such as Solanine (Sigma) as internal standard for quantification (standard), and quality control for HPLC runs and extraction efficiency etc. as well.
http://www.sigmaaldrich.com/catalog/product/aldrich/s3757?lang=en®ion=US
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