Which type of base is generally used in Heck Coupling and why?

More Marium Akram's questions See All

How to calculate conductivity using Electrochemical Impedance Spectroscopy's Nyquist plot?

Hello everyone, I am new to this characterization but watching videos on youtube I have already plotted a Nyquist plot (Z' and -Z'') and already fitted models on my material using Zsim. But now I...

29 July 2024 1,376 0 View

How should we increase the quality of RNA extraction?

I’m having difficulty achieving high RNA integrity in my samples. Although the 260/280 and 260/230 ratios are satisfactory after RNA extraction, the RNA samples show signs of degradation when...

22 July 2024 155 4 View

What is the angular difference between two sets of Euler Angles?

Let's say I rotate a hexagonal crystal using Euler angles (φ1, φ2, φ3); and rotate another similar crystal using Euler angles (ψ1, ψ2, ψ3). What would be the angular difference between the final...

17 July 2024 5,582 1 View

Why yeast transformation is not working?

I have been trying to delete one copy of an essential gene from a diploid in fission yeast. I am using PCR based homologous recombination method and primers are designed from Bahler's lab website....

12 May 2024 1,274 4 View

What is the breakpoint diameter for florfenicol and oxytetracycline in the disk diffusion?

What is the breakpoint diameter for florfenicol and oxytetracycline in the disk diffusion method, indicating susceptibility or resistance to these antibiotics in the tested salmonella strain?

25 April 2024 5,168 1 View

What's the role of Carbon Black in the formation of slurry for electrochemical study and what alternative we can used in place of carbon black?

For Electrochemical study, slurry is formed to test the material in three-electrode system. Why we need Carbon black for this slurry formation and what it's alternatives?

17 March 2024 1,531 0 View

What are the fundamental differences between agar well diffusion and disc diffusion methods? Which one can provide more precise findings?

Hi everyone, When evaluating the antibacterial activity of antibacterial agents, there are two methods commonly mentioned in the research articles, which are agar well diffusion and disc...

16 March 2024 4,332 10 View

What is the best solvent to get the crude extract of plants' leaves, and at what concentration could the solvent be, generally?

Hi everyone, I need to know what solvents are commonly used, the type of solvents, their purpose, and their recommended concentrations. Any advice is welcome. Thank you.

03 March 2024 9,969 4 View

Proving that the odd natural numbers can all be expressed with a specific form is equivalent to the proof of the Collatz conjecture. Easy enough?

My new article about the Collatz conjecture proves that a simple arithmetic statement is equivalent to the proof of the Collatz conjecture. The proof of the Collatz conjecture is equivalent to...

21 February 2024 6,670 0 View

Overview of Machine Learning. What are the opportunities it brings to developers? What threats does it pose to society?

I need a referenced cites on the above

17 February 2024 7,991 2 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

Why my colony PCR results of my recombinant bacterial not showing any results?

I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....

05 August 2024 2,570 3 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View

PCR showing no bands - Master mix and primers didn't mix?

Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...

31 July 2024 2,406 6 View

Dimensions of an MJ Research 96-well alpha module?

Can anyone with an MJ research / BioRad PCR machine from ~2010 or earlier tell me the external measurements (LxWxH) of the removable standard PCR alpha modules that can be removed from a PTC200 or...

30 July 2024 2,867 0 View

How to retain amplicon yield after Ampure bead cleanup, following a 2-stage PCR protocol?

Hello all, I have been trying to follow a 2-stage PCR protocol used to amplify barcodes of a large yeast library, as per Nyugen et al. (2022) -...

30 July 2024 841 2 View

Someone have the key or the installer of the program Mx pro 3000 (PCR real time)?

I need to install this program

25 July 2024 4,756 0 View

What is the right voltage sweep direction for characterization of HER kinetics?

In general, when performing a Tafel analysis for corrosion resistance measurements, a line voltage scan is performed from say -250 to +250 mV vs. OCV at low scan speed around 0.125 mV/s. However,...

25 July 2024 7,982 3 View

Do you mix and add all primers together in a singel PCR reaction (4 total primers for 2 mutations) when using Agilent's multi-site mutagenesis kit?

I want to introduce 2 mutations using Agilent's multi-site mutagenesis kit, I designed the primers using their online tool and it gave me 2 sets for primers (1 set for each mutation) so I was...

25 July 2024 6,517 3 View

Illustra™ MicroSpin™ G-25 columns what it is used for?

Hello, We found three packages of Illustra™ MicroSpin™ G-25 columns in the cabinet of an unused lab. They are very old but have never been opened. I have never used this kit before, and I couldn't...

25 July 2024 4,927 3 View