Which transformation method is suitable for microalga Chlorella Protothecoides and gives high transformation efficiency?

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Sadib Bin Kabir

Chlorella species contain Algenans in their cell wall. So anaerobic digestion without pretreatment would be difficult. I think you might go for bioethanol generation. Also, it contains a large fraction of lipids. If you grow it in nitrogen stress medium it will contain up to 55% of lipid, so the biodiesel conversion through the transesterification process would be also economical.

Vijita V. Pillai

See if any of these links can be of help to you:

Article Rapid and efficient genetic transformation of the green micr...

Article Development of a New Method for Genetic Transformation of th...

Article Engineering a malic enzyme to enhance lipid accumulation in ...

Good luck!