So i have a study with both males and females, they will both be shown a negative image and their brain reaction recorded and a positive image with their brain reaction recorded
Anita Hutchinson I think you are trying to test two hypothesis in one go. Either you can do a gender neutral analysis or a stimuli neutral analysis.
Other way to go about is to use logistic regression. Here the response I assume is positive or negative reaction. So the dependent would be reaction, while predictors would be the gender and the type of imagery. It will give a likelihood equation, and odds ratios. Interactions can be calculated if your study indicates such. The end result will form a Y = m1x + m2x + C equation.
It is clear that you are seeking the reactions of both males and females when they are shown a positive image and a negative image. However, it is not clear whether the reactions were sought in terms of pre-decided categories or the reactions as given by them. If the reactions were recorded in structured format then the analysis can be easy otherwise more efforts are needed.
To make my point clear, consider a situation where we have designed a banner for our club and wanted to know how it is liked by the members. One situation is we record the answers as given by them while another situation could be we give them the choice between pre-decided answers (may be on 5 point scale) like:
1. Very bad; 2. Bad; 3. No comments; 4. good; 5. Very good. In case of situation 1, you have to carefully categorized the answers into more than one class based on the frequency analysis of the answers. So, please make it clear, how did you took the reactions. Was it open or was it in pre-decided categories?
Anita Hutchinson you may need to provide more information on your data processing. If you want to analyse brain responses (i.e. EEG, fMRI, ... ), SPSS may not be the best tool for the job (depending on where you stand in your analysis progress). What type of data have you collected and have you done any baseline subtraction, averaging, etc.? Brain responses usually have a temporal progression. Do you want to analyse the "whole" brain response or do you have any specific hypotheses? Statistics in neuroscience often come with special adjustments regarding multiple comparisons and alpha adjustments - you may need to look into some journal papers to get a sense of the typical analysis.