How to identify whether a particular bacterial gene is chromosomal or on plasmid, before sequencing?
Extract plasmid and transform it to competent cells. With the help of presence or absence of gene product in the transformed cells, we can see whether the gene is in plasmid or chromosome
Do you have any information about flanking sequences? Otherwise you could use FISH.
Perhaps Southernblot hybridisation of chromosomal an plasmid preperations will help you to be sure about the localisation of your gene. This is a quite time consuming technique so you had to calculate if you surely need this information.
Extract plasmid and transform it to competent cells. With the help of presence or absence of gene product in the transformed cells, we can see whether the gene is in plasmid or chromosome
I'm afraid there is lack of information to really answer this question. In which bacterium are you working? E. coli? Do you know the gene sequence or its family?
Like Joshua proposes Southern blot will always work, but there are easier ways if you have more information.
actually i have isolated the bacteria from soil. these were then grown media with different concentrations of heavy metals.
Can plasmid curing be of any help?? how efficient it can be?
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