Which solution do you use to treat encrusted pyelitis (corynebacterium urealyticum)?

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What are the potential complications associated with uncontrolled hyperglycemia in the perioperative setting?

What are the potential complications associated with uncontrolled hyperglycemia in the perioperative setting?The Impact of Hyperglycemia on Perioperative Outcomes in Pat...

07 May 2024 4,190 1 View

The following three papers are widely cited by geodesists, but they are all written in German; can anyone help with translating them into English?

Meissl P (1962) Die innere Genauigkeit eines Punkthaufens. Österreichische Zeitschrift für Vermessungswesen 50, Nr. 5, S. 159–165, Nr. 6, S, pp 186–194 Meissl P (1965) Uber die innere Genauigheit...

04 May 2024 6,178 0 View

Would like to learn about Auguste Levasseur, Lafayette's friend and author of the book on their visit to the United States in 1824-25?

The Marquis de Lafayette is "returning" to America again this fall. After serving in the American and French Revolutions, he returned to America in 1824-1825 and toured some 25 states. This...

19 April 2024 4,979 0 View

Partial trace over a finite system or finite-approximation of an infinite system, which one is preferable?

When dealing with composite systems comprising a finite subsystem and an infinite one, the aim often involves numerically analyzing specific properties, such as entanglement entropy between these...

02 April 2024 1,640 1 View

Is there any simple online model for pesticide environmental and human risk analysis using questioner ?

model methodology analysis inputs out puts and other related issue

17 March 2024 1,733 1 View

Can any one give Special properties and examples of Graphs that are (i) Uniquely (ii) doubly (iii) triply k- dimensional?

Let M = {v1, v2 ... vn} be an ordered set of minimum number of vertices in a graph G. Then the vector of distances (d(u, v1), d(u, v2) ... d(u, vn)) is called the M -coordinates of a vertex u of...

24 February 2024 3,228 2 View

How can I determine and know the doubling time PDT for MCF-7 and MDA-MB-231 cell lines? Explain answer as much as possible please ?

I mean.. is there a specific time for doubling time for each cell line or it is different and depend on the experiment ?

23 February 2024 8,508 3 View

Is there any deterministic algorithm to find Metric dimension of any graph in a reasonable time?

In our Research Journey, an algorithm and software coding were revealed to me. It is useful to find all metric bases of a given graph in a reasonable time. For some graphs , we get all metric...

06 February 2024 5,067 6 View

Best way to fit mosquito incidence model with future climate data?

I have tried with no avail to make a logistic regression and random forest model work. First i fitted a model with climatic measurements as independent variables (2015-2020, almost five years)...

10 January 2024 9,738 1 View

Competition Assay of peptides in flow cytometry?

Hi, I performed a competition assay of serially diluted concentrations of the unlabeled peptide by adding a constant concentration of the FITC-labeled peptide. I have observed higher fluorescence...

06 January 2024 6,949 6 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

The question is how to use Wavenet transform?

HOW CAN I WRITE A CODE TO USE THE WAVENET TRANSFORM AS A FEATURE EXTRACTION METHOD INSTEAD OF DWT IN MATLAB?

03 August 2024 7,829 0 View

Transfection in HEK293T cells?

Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...

31 July 2024 9,892 4 View

Can i use the protease inhibitors during cell membrane vesicle preparation ？?

I am currently working on a project that involves extracting cell membranes, for which we disrupt the cells using sonication. During the initial extraction process, we add protease inhibitors to...

30 July 2024 7,077 1 View

How to retain the a GFP tagged gene expression in stable cell line?

Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....

29 July 2024 5,007 0 View

What is the relationship between protein structure and N or C terminal tagging choosing?

I want to do 2,3-butanediol dehydrogenase(BDH) enzyme purification to confirm its activity for 2,3-butanediol. Before that, I need to confirm which N or C terminal tagging is better for enzyme...

28 July 2024 366 3 View