How to report results from a multinomial logistic regression?

I have run a multinomial logistic regression and am interested in reporting the results in a scientific journal. Would it be alright to include a model summary table with the coefficients,...

01 June 2020 4,107 12 View

Would monoclonal antibody aggregates effect the absorbance during a Bradford Assay?

I have purified an IgG from cell supernatant using a Protein G column. Following this size exclusion chromatography was used to determine aggregates of the IgG, monomeric antibody and antibody...

08 November 2019 1,487 3 View

Amino acid numbering: first amino acid of the mature or nascent protein?

Hi, Could anyone chime in on amino acid numbering for proteins with a secretory signal? Should the numbering start from just after where the SS gets cleaved off, or from the first methionine? I...

31 May 2017 5,405 5 View

How does teenage motherhood affect the cognitive outcomes and academic achievement of teenage mothers's children?

17 March 2017 5,810 2 View

QPCR melting curve analysis shows a shoulder on BOTH sides of the curve. What does this mean ?

11 January 2017 2,475 4 View

Why is the background signal on my Western blot not improving?

I have been performing a Western blot where I probe for my protein of interest, then strip and reprobe for the loading control (I haven't been blocking after stripping, just incubating in primary...

13 May 2015 7,484 17 View

Should I ligate my two DNA fragments together first and then ligate that construct into my plasmid? Or throw all three together in at the same time?

I have two fragments one is 2.9kb the other is 2.7kb. I need to ligate those two fragments together and then ligate my new construct into a 4.36kb plasmid. Should I do two separate reactions, one...

16 March 2015 8,755 13 View

Is there a SV40 large T-antigen encoding region for sale already ligated into a plasma that I could purchase somewhere?

I am trying to create a construct consisting of the GnRH promoter region from a chicken and the SV40 large t-antigen (TAg) encoding region for my master's thesis (to later create an immortalized...

05 March 2015 2,846 2 View

Stats - how to compare two things for significance to see if one is more valid than the other?

I'm comparing two adherence diary designs to an objective measure (an accelerometer - all will measure how much participants walk each day) and I want to assess how closely each diary relates to...

11 September 2014 4,176 6 View

Has anyone used the phospho S6 ribosomal protein antibody for western blotting in vitro?

I am trying to find out if people use the Ser 235/236 site or the Ser 240/244 site for the S6 ribosomal protein antibody? I am working in vitro using skeletal muscle and need to decide which...

01 April 2014 6,016 3 View

Where can I find life cycle inventory or assessment of non woven materials?

Dear network, I am looking for the LCA or LCI or at least the energy balances of the non woven fabrics process for my master project, in specific for natural fibers, yet I have found...

02 March 2021 9,662 1 View

Please suggest a good text book for "Research Methodology in Life Sciences". Which book would be good for PhD Scholars?

Please provide Book Title and author name

02 March 2021 9,059 3 View

Is Journal of Agricultural Research a predator?

it is published by Juniper

28 February 2021 354 4 View

How can plant phytochemistry be included in discussions about whole plant traits?

I am using the concept of plant functional traits (Cornellisen et al., 2003) in a paper. Considering descriptions for whole plant traits (life history; life form; growth form; plant height;...

24 February 2021 6,831 5 View

Has anyone tried to scan fluorescence IHC slides with microarray scanner?

I have an old slide microarray scanner (VIDAR Revolution 4200) and would like to give it a "second life", as IHC scanner array (fluorescence dyes), FISH over tissue or similar. Has anyone tried...

24 February 2021 9,210 1 View

How to read a flow cytometry graph detailing cell cycle?

Hello everyone, When determining cells in different stages of the cell cycle, cells are stain for Brdu and Propidium Iodide. I understand how PI can be used to discriminate between G1 and S/G2...

18 February 2021 3,920 3 View

What to do with absence of fluorescence label in Fucci2a mouse-derived fibroblast culture?

Dear all, I have harvested fibroblast cells from R26p-Fucci2 mouse embryos (mouse line as described in this paper: https://dev.biologists.org/content/140/1/237) and cultured them on plastic,...

17 February 2021 3,368 2 View

Where can I obtain agricultural land use data for Wales, UK?

The Gower peninsula around Swansea is my target area

16 February 2021 7,473 3 View

How to find orthotropic material properties?

I am doing an impact analysis of bullet on a plate. But for that simulation, I require orthotropic properties of materials like PPS and PEEK which are Unidirectional type. How to find them?...

16 February 2021 8,775 2 View

How can we assess applied digital skills and soft skills through online examinations during COVID-19 semester?

The final qualification assessment for major programs is a strict regimen that involves different stages - oral and written exams, final project viva, internal and external review. How are ICT...

16 February 2021 1,762 8 View