Glass-ceramics are generally less bioactive than their glass counterparts. A range of SBF solutions is available to check in-vitro bioactivity such as SBF,c-SBF, r-SBF, Tas-SBF, TRIS etc. Which one is most suitable if the samples have low bioactivity?
ynthesized glasses, the ability for apatite formation on their surface upon their immersion in simulated body fluid (SBF) was checked using SEM and pH measurements. MTS assay cytotoxicity assay and live-dead cell viability test were conducted on J774A.1 cells murine macrophage cells for different glass concentrations. silicon and calcium in the SBF and subsequent formation of calcium phosphate, 27-Tris-SBF is best to asses the in-vitro apatite-forming ability using pH measurements, XRD and SEM.aqueous phosphate solutions. glass/glass-ceramic soaked SBF.
The ionic composition of the (most used) SBF and HBSS solutions, compared to human plasma, can be found at Table 1.5 (p. 36) of the reference given below. For preparing the mentioned SBF solution, you may check Table 5.1 (p. 206) at the same reference. For alternative modified SBF solutions, you may want to check references quoted herein (p, 36).
Thesis Cristalização de Biomateriais Vitrocerâmicos e Mineralização...
Prof. Carlos Araújo Queiroz, Thanks for your answer and shared thesis.
The thesis is in written in Portuguese. I could not read text much, but the tables you mentioned are of great interest for me. Also I have got much help from your other publications.
Thanking you again,
Satwinder Singh.
Thanks Dr. Upadyay for your kind attention and suggestions about my question.
Hi Satwinder,
To answer your question, SBF is not an ideal medium to study the bioactivity of glasses. There is enough literature to prove that. You may choose any SBF, all of them are essentially buffer solutions rich in calcium and phosphate ions, with more or less similar compositions. If you leave SBF on its own, you will observe calcium phosphate precipitation at the bottom of flask after few days.
Having said that, it doesn't mean that I have not used SBF for my work. Even I have done it in past. I have used Tas-SBF for most of my work. However, I have also corroborated my work with understanding chemical dissolution of glasses in other aqueous media devoid of calcium and phosphate.
What is really important to study is the chemical degradation of your glasses in pH relevant to human body plasma. For example, start with studying the chemical dissolution behavior of glasses in deionized water.
I hope this helps.
Ashutosh
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