We are designing a strategy to make CRISPR knock-in intestinal organoids using Lentiviral delivery (as other transfection method are very inefficient in intestinal organoids). For designing the guide RNA, this seems straightforward, and we are planning to use the lentiCRISPRv2. However, for the repair template we are struggling to figure out which backbone would be best. The worry is that cloning our repair template with homology arms into a constitutive Lenti plasmid would result in random insertions of the homology arms and tag instead of being used as a repair template. Does anyone have experience doing CRISPR knock-ins using lentiviral delivery that might be able to give some advice? Thanks!