We have to determine easily-extractable glomalin (EEG) and total glomalin (TG) from soil samples, but we have found various contradicting methods and calculations. Is there a trusted method that can be used to yield more consistent and trusted results?
Thank you in advance for your kind assistance.
@ Johan, the parameters of the NIRS calibration model (R = 0.90 for soils from arable land and grasslands and R = 0.94 for forest soils) proved that glomalin can be determined in air-dried soils by NIRS with adequate truthiness and precision.
You can use the Bradford method for proteins.
It is a method of successive extractions with sodium citrate. Although there are authors who have modified some variables for some soil relationships. It is advisable. Accuracy and precision will depend on laboratory work.
Wright, S. and A. Upadhyaya. 1998. A survey of soils for aggregate stability and glomalin, a glycol-protein produced byhyphae of arbuscular mycorrhizal fungi. Plant and Soil 198: 97-10
By using near-infrared spectroscopy and soil samples were extracted by sodium citrate solution (pH = 8.00) following the procedure described by Wright and Upadhyaya (1996).
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