In our case, prey might be both terrestrial and aquatic so I need primers that amplify at least:
- Diptera (including chironomidae)
- Lepidoptera
- Coleoptera
- Collembola
- Trichoptera
- Plecoptera
- Ephemeroptera
... without amplifying spiders !
Of course, I have already selected LCO 1490 / HCO 2198... But maybe there are other options...
Hello Denis,
I'm interested to know how you will manage to not amplify spiders with LCO1490/HCO2198 primers ?
Hi,
Sorry I made a mistake, I wanted to say LCO 1490 / MlepR1 which as been used for coastal spiders gut content. I must also add that I will target Pardosa species which are not amplified by this pair.
My problem is that I do not know if it is efficient for Trichoptera, Plecoptera and Ephemeroptera.
Tharaka, thank you for the useful comment, I will have a look at the paper...
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