Hello everyone, I wonder if you want to help me with doubts about fluorescence intensity quantification and cell comparison.

I am analyzing the fluorescence intensity of lysosomes in skin fibroblasts. To do that, I select the whole cell as a ROI in Fiji. The program gives me several parameters for fluorescence intensity, such as mean, Indent, and RawIndent. I do not know which parameter to use to compare the fluorescence intensity between groups. Some people use Indent/Area, others mean/area or CTCF.

I wonder If you can help me with that problem?