I am using spectrophotometric method for the quantification of the drug. The lipids interfere with the absorbance. I am unable to use Triton X to lyse the liposomes as Triton X also absorbs at the wavelength of my interest.
To quantify the drug in anionic vesicles like SDS..same procedure can be followed to dissolve the pellet ..either chloroform or methanol..for accurate results..make a calibration curve of the drug using the solvent which is used to dissolve pellet
It would help to know what the liposomes are made from. If they are made from phospholipids, you might be able to use a phospholipase C in very small amounts to separate (hydrolyze away) the polar head group from the nonpolar tail(s) and then use chloroform to separate the nonpolar tails from the water-soluble drug.
https://en.wikipedia.org/wiki/Phospholipase_C
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