How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

No title

03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

What are your alternative data collection methods during the pandemic? What are the pros and cons?

Dear colleagues We are exploring possible data collection methods for environment and behavior research in the context of architectural environments. Have you conducted any Post Occupancy...

16 February 2021 2,575 4 View

Any way to separate out fluorescent channels from a composite image?

Hi there, I've been using the EVOS Auto II microscope for some time now and usually I will save the transmitted light/bright field image and the images from fluorescent channels as separate...

15 February 2021 546 3 View

Centrality indices for weighted bipartite networks?

My colleague and I are looking to use SNA to look at the risk of contact between an intermediate and definitive host of a parasite. We have built a bipartite network where one set of nodes...

08 February 2021 6,637 3 View

How should I go about evaluating cytoplasmic and nuclear Arginase-1 activity?

Hello! I've stained some bright field Arginase-1 Hematoxylin slides, but I'm having a hard time finding any computed histology predicates for evaluating the intensity of the cytoplasm verses the...

03 February 2021 3,039 1 View

What is the best way to minimize/prevent the effects of rigor mortis in excised mouse muscle?

I'm planning to excise mouse muscle to use for ex vivo mechanical testing. What is the best way to prevent the stiffening effects of rigor mortis from confounding results if I cannot isolate the...

30 January 2021 1,641 2 View

When is it optimal to choose an OFAT (One factor at a time) design over DOE (design of experiment)?

As a current undergraduate student majoring in Microbiology who is still learning DOE (Design of experiment) on my own, I have heard and read that an OFAT design is considered the most inefficient...

29 January 2021 8,256 3 View

What is the harmless way of cutting epoxy filled nanocomposite ?

I want to cut epoxy filled nanocomposite for FE-SEM analysis. What is the best way to cut it without resulting in any cracks or change in position or alignment of the particle reinforcement ? Also...

28 January 2021 7,084 5 View

Can I use several qualitative traditions at the same time for my Master thesis?

Hello, I am doing a research paper in which I will have to do semi-structured interviews to a group of SMEs from my hometown to analyze and define their needs and interests. Which tradition of...

27 January 2021 6,140 3 View

Lighting for open field test behavior assay?

I am currently setting up our lab's behavioral space to run the open field test in rats, and I am trying to figure out how to set up lighting to evenly illuminate the open field chamber (a 90 cm x...

26 January 2021 1,399 3 View

Why sometimes current- voltage plot in chemiresistors/ FET does not pass through the origin?

I am trying to measure resistance from I-V curve from a chemiresistor/ FET sensor with a keithly sourcemeter. The substrate is soaked/ wet with protein and phosphate buffer only. But the I-V line...

24 January 2021 8,009 3 View