Which of these techniques is both more sensitive and more reliable for the detection of Mycoplasma contamination in issue culture?

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Which of these is an efficient strategy to get rid of a Mycoplasma contamination in a cell culture?

Anyone who can help me with this question? a. Wash the plate several times with fresh medium previously filtered through a 0.4 ?m filter. b. Wash the plate several times with fresh medium...

01 February 2019 6,401 4 View

What is the most important reason for eradicating Mycoplasma from your tissue culture?

a. Mycoplasma affects many cell parameters leading to erroneous and irreproducible experimental results that will affect the quality of your publications. b. Medium becomes turbid and cells are...

01 February 2019 3,321 2 View

If I receive a new cell culture or a frozen cell line from one of my lab fellows or from a colleague in anoth. lab what should I do first?

Anyone who can help me with this question?! a. Always start culturing the cell line in the quarantine incubator, not sharing the culture medium with your colleagues, and test it for Mycoplasma...

01 February 2019 4,620 7 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

I want to performe MALDI-TOF MS for bacterial identification, I would appreciate it if anyone can tell me the matrix solution preparation protocol ?

based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...

26 February 2021 6,799 3 View

Is it possible to have two luminescence bands of the same peak position but with different intensities in single spectrum??

Hi! If it is possible to have two luminescence bands of the same peak position in a spectrum, what is the reason behind formation of two separate bands instead of one? This phenomenon is related...

26 February 2021 4,963 5 View

What is the best concentration of cDNA to be used for qPCR?

I have synthesised cDNA from 500ng/ul of RNA. The concentration of the cDNA Is about 5000ng/ul. Should I dilute my cDNA or just use it as it is for my qPCR? I usually use 2ul of cDNA for a 20ul...

24 February 2021 8,618 3 View

Recommendations on homogenizers for RNA isolation?

I will be performing RNA isolation with frozen lung biopsy samples and am in the market for a tissue homogenizer. I am wanting a homogenizer that will fit in a 1.5ml tube. I was looking at the...

24 February 2021 3,623 3 View

Alireza Mordadi

All methods mentioned by you are very sensitive and reliable.

Cheapest and easiest way is PCR.

Samuel A. DeCero II

16s rDNA detection using PCR...primers designed to the v3-v4 region.

Mehdi Rostami Rad

Efshan Babazade

Naila Altaf

PCR

Sushil Kumar

Both the techniques that you mentioned above are sensitive and reliable any of them you can use