Which of these techniques is both more sensitive and more reliable for the detection of Mycoplasma contamination in issue culture?

More Efshan Babazade's questions See All

What is the most important reason for eradicating Mycoplasma from your tissue culture?

a. Mycoplasma affects many cell parameters leading to erroneous and irreproducible experimental results that will affect the quality of your publications. b. Medium becomes turbid and cells are...

01 February 2019 3,393 2 View

Which of these is an efficient strategy to get rid of a Mycoplasma contamination in a cell culture?

Anyone who can help me with this question? a. Wash the plate several times with fresh medium previously filtered through a 0.4 ?m filter. b. Wash the plate several times with fresh medium...

01 February 2019 6,496 4 View

If I receive a new cell culture or a frozen cell line from one of my lab fellows or from a colleague in anoth. lab what should I do first?

Anyone who can help me with this question?! a. Always start culturing the cell line in the quarantine incubator, not sharing the culture medium with your colleagues, and test it for Mycoplasma...

01 February 2019 4,685 7 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

Separation of organic acids-HPLC?

Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.

07 August 2024 8,782 3 View

E.coli contamination in human RNA seq data ?

Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...

06 August 2024 807 3 View

RNA later for the preservation of RNA in fecal samples at room temperature for one day (37°C)?

I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...

06 August 2024 1,367 3 View

The aqueous fraction of the hydroethanolic extract is showing the presence of palmitic acid. What is the mechanism responsible ?

Palmitic acid presence in aqueous fraction

05 August 2024 8,624 4 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

How to convert g/kg Humic acid dose to kg/ha?

I used humic acid at 0.044 g/kg soil in my pot experiment. But finally, I have to recommend kg/ha. Each pot's soil weight was 11 kg. What is the solution?

02 August 2024 7,186 6 View

Alireza Mordadi

All methods mentioned by you are very sensitive and reliable.

Cheapest and easiest way is PCR.

Samuel A. DeCero II

16s rDNA detection using PCR...primers designed to the v3-v4 region.

Mehdi Rostami Rad

Efshan Babazade

Naila Altaf

PCR

Sushil Kumar

Both the techniques that you mentioned above are sensitive and reliable any of them you can use