Efshan Babazade

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Questions related from Efshan Babazade

What is the most important reason for eradicating Mycoplasma from your tissue culture?

a. Mycoplasma affects many cell parameters leading to erroneous and irreproducible experimental results that will affect the quality of your publications. b. Medium becomes turbid and cells are...

02 February 2019 3,369 2 View

Which of these is an efficient strategy to get rid of a Mycoplasma contamination in a cell culture?

Anyone who can help me with this question? a. Wash the plate several times with fresh medium previously filtered through a 0.4 ?m filter. b. Wash the plate several times with fresh medium...

02 February 2019 6,474 4 View

If I receive a new cell culture or a frozen cell line from one of my lab fellows or from a colleague in anoth. lab what should I do first?

Anyone who can help me with this question?! a. Always start culturing the cell line in the quarantine incubator, not sharing the culture medium with your colleagues, and test it for Mycoplasma...

02 February 2019 4,660 7 View

Which of these techniques is both more sensitive and more reliable for the detection of Mycoplasma contamination in issue culture?

a. Mycoplasma ribosomal RNA (rRNA) detection by nucleic acid hybridization using a luminescent probe. b. PCR of mycoplasma DNA. c. Flow cytometry based on DNA content.

02 February 2019 7,447 5 View