Dear Lensi N Patel , generally the phytohormones are extracted from the plant material using solvent ( you can use sowhlet extraction or ultrasound assisted extraction), then the samples are dried with rotavapor and analysed by LC-MS or HPLC, recently a lot of studies uses UPLC and multidetection techniques, check the references below, I hope they help you:
best regards
I wish I could help, I will read the rest of the answers.
Dear @Lensi N Patel Please also check the links, and the attached PDF:
https://www.researchgate.net/publication/333758079_Identification_and_quantification_of_some_phytohormones_in_seaweeds_using_UPLC-MSMS
https://www.hindawi.com/journals/jchem/2020/4910621/
https://link.springer.com/article/10.1007/s00344-018-9816-9
Best wishes, AKC
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