Which methods are you using?

More Caleb Ritenour's questions See All

LC3 bands are showing up in the wrong size range?

Hi all, I have been attempting to blot for LC3I and II and i have been having a strange issue, the bands seem to be showing up around 23 kDa. I have done the blot twice now and still the same...

28 March 2024 3,621 5 View

How to handle twins in birth order analysis?

Hello! I have a methodological question and I hope someone can help me. I collected data via an online survey with a sample size of 2600 (N = 2600). Initially, I aimed to examine the effect of...

26 February 2024 6,911 4 View

Has anyone heard of conjugating BioId to a lipid (LPS)?

I know BioID typically has to be spliced into a gene of interest. But my project is trying to identify host cell proteins interacting with LPS. I am having trouble finding methods for doing a wide...

31 August 2023 5,746 0 View

Equation for Fluorescent Displacement Assay of Competitive Inhibitors?

I am currently trying to develop an assay to test the binding affinity (Kd) of a binding inhibitor to its target, through the displacement of a fluorescent ligand that is pre-bound to the target....

17 April 2023 7,694 1 View

Why is my DSC peak negative?

I have been told that because the DSC peak represents heat capacity (Cp) it should and cannot go below the zero mark as the temperature increases. But I have curves that go below the zero mark -...

15 February 2023 9,891 6 View

I would like to ask if anyone here might be able to provide me with a blue swimming crab fishery in the Bicol Region for my undergraduate thesis?

I am currently undertaking my undergraduate thesis entitled: Characterizing the Portunus pelagicus (Linnaeus, 1758) Fisheries in Bicol Region and the Possible Impacts of Illegal, Unreported, and...

11 September 2022 9,322 3 View

What are the current national policies implemented to prevent further instances of Illegal Unreported Unregulated fishing in the Philippines?

I wish to conduct research on Illegal, Unreported, and Unregulated fishing and poaching of mobulidae species in Jagna, Bohol, Philippines.

16 March 2022 9,577 2 View

Hello. Is there any possibility of using Random Forest Model to predict the possible features of a specified target?

I used a Random Forest regressor to predict the concrete compressive strength of mixtures with good accuracy. Now, I want to use the Random Forest regressor model to generate possible features...

09 August 2021 6,966 2 View

Purified protein requires Calcium for activation, but the elution buffer I used seems to interact with Calcium, help?

I am working on a project where I have to purify some proteins that are tagged with a 6x His tag. Since my proteins were produced by bacteria, I purchased the HOOKTM 6X His Protein Purification...

09 February 2021 7,235 4 View

Can someone explain blocking vs. nonblocking electrodes in terms of electrochemical impedance spectroscopy?

I have fabricated thin films and want to measure the impedance parallel to the material. I suspect the films are conducting protons and electrons based on some Hall effect measurements I have...

27 January 2021 2,108 5 View

How are iso-frequency contours plotted?

Let's say we have a standard, regular hexagonal honeycomb with a 3-arm primitive unit cell (something like the figure attached; the figure is only representative and not drawn to scale). The...

07 August 2024 1,937 1 View

Why do i have a weak plasma and no coating during rf sputtering?

I have been getting a low plasma and no coating while doing rf sputtering of copper doped ZnS using a power of 140 watts and at pressure of 6.5x10-3 Mb.

07 August 2024 4,526 4 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

Broca’s area must be intact for the learning of new movement sequences?

When the eyes of a person are damaged this causes complete blindness. Likewise, when Wernicke’s and Broca’s areas of neocortex are damaged this causes complete aphasia, losing the ability to...

01 August 2024 6,744 2 View

The Origin of Human Language?

I attended a lecture at the Baylor College of Medicine (~ 2019) where one of the questions was “Does birdsong have anything to do with human language?” Noam Chomsky would say, “Absolutely not!”...

31 July 2024 1,706 4 View

Creating an Automaton/Using Language as the Model?

As animals learn a task, they become more reliant on their long-term memories as compared to the real-time sensory information to guide behavioral performance (Ahilan et al. 2018). This process...

31 July 2024 9,859 0 View

What exactly is RAG-LLM doing? Isn’t it data engineering?

What exactly is Retrieval Augmented Generation for Large Language Model doing? Isn’t it data engineering?

30 July 2024 7,376 3 View

Why only alpha wave of brain are found in some patients?

I have found an EEG where only alpha waves are present. Beta waves are not found in active patients. What interpretations ?

26 July 2024 4,741 1 View

How to select relevant metabolites from multiple annotations ?

I am using the Polyomics Integrated Metabolomics Pipeline (PiMP) for metabolite feature analysis. After annotating my data, I have encountered cases where multiple potential metabolites are...

24 July 2024 5,789 0 View

What are the strategies to Enhance IgG-Producing Plasma Cells in mice for Monoclonal Antibody Development?

I have immunized BalB/C mice with a protein using the intradermal (ID) method with Complete Freund's Adjuvant (CFA) and Incomplete Freund's Adjuvant (IFA), following a 14-day interval and three...

22 July 2024 9,160 2 View

Csaba Ferencz

The Method of Inhomogeneous Basic Modes. You can find the details in my papaers and books.

H. L. Pécseli

What is best, depends on your problem. Most researchers are quite happy with spectral codes (usually for periodic systems), but if you are interested in large amplitude problems, shocks etc., you might prefer SPH-codes. as summarized for instance in:

The Astrophysical Journal, 652: 1306Y1317, 2006 December 1

MULTIDIMENSIONAL MHD SHOCK TESTS OF REGULARIZED SMOOTHED PARTICLE HYDRODYNAMICS

S. Børve, M. Omang, and J. Trulsen