1.Need a standard method , which proceed with removing of inorganic carbon.
2. I need titration based method due to facility issues.
I have a method but could not reference of that one, method is -
1) 1 g soil
2) then add 10 mL 6 N HCl to remove the inorganic carbon
3) Wash with water and dry it.
4) after that follow walkley and Black method.
The determination of soil organic carbon is based on the Walkley-Black chromic acid wet oxidation method .
determination by combustion using temperatures above 900 °C with prior acidification is an established technique included in many standard methods, e.g. EN 15936. TOC determination is a widely used method to determine the volume of humus and organic material in soil and waste.
I did a little review in the area is and is as below: Article Developments in Fractionation and Measurement of Soil Organi...
3.1. Wet Oxidation and Dry Combustion Methods
The most commonly used methods for determination of SOC are wet oxidation by Walkley-Black method [27] and dry combustion by LECO CN Analyzer [28]. The latter method estimates SOC more accurately. Due to incomplete oxidation of SOC in a dichromate-sulphuric acid mixture, the Walkley-Black method underestimates SOC concentration when no heating is applied. To over- come the problem of incomplete C recovery, which leads to the underestimation of C concentrations by Walkley- Black method, the analytical results of this technique are adjusted by 1.32 as a correction factor assuming the re- covery rate of 76% [29]. However, modified dichromate oxidation techniques that involve extensive heating do not require a correction factor because most of the or- ganic C in the soil is oxidized to CO2 [27]. Some authors reported that the recovery of 76% is generally too large [30,31] and that it may vary according to land use, soil texture and sampling depth [30-32].
3.2. Chemometric Statistical Methods
With the development and emergence of reflectance spe- ctroscopy techniques, near infrared (NIR) and mid-infrar- ed (MIR) are also used to determine SOC in the labora- tory. Using chemometric statistical methods, both NIR and MIR spectroscopy techniques have been used in the past two decades to determine soil properties, including soil organic C and total nitrogen [33-35]. Compared with wet and dry combustion methods, the NIR and MIR spectroscopy techniques are faster, robust, cheaper and non-destructive, and they do not require any reagents for analysis [36,37]. These emerging techniques are, there- fore, likely to replace the tedious job of wet and dry combustions in future, and are highly attractive for stud- ies where a large number of analyses are required [34].
3.3. In Situ Methods
The techniques used for in situ measurement of SOC in the field are by sampling, or by on-the-go detection with sensors mounted on a tractor [38,39] that facilitate instant measurement of SOC. These methods are also non-de- structive and relatively cheaper compared to the destruc- tive wet laboratory [27] or dry combustion [28] methods. Remote sensing is another tool for in situ measurement of soil properties in the field [40,41] based on spectral laboratory data and this method captures large spatial area unlike the other previously mentioned techniques. The other in situ methods include Laser-Induced Break- down Spectroscopy and Inelastic Neutron Scattering techniques [41]. A recent review covers more detail on SOC dynamics with fractionation and measurement tech- niques [3].
Thank you
Dear Rajendra,
Please kindly find the attachment. This may be helpful to figure out your query.
All the best.
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