I am going to use SOM for my adsorption experiments. I know the procedure of standard SOM purification from IHSS. However, is that method popular utilized for SOM purification?
I am providing a link for a paper on Innovative Methods for Soil DNA Purification Tested in Soils with Widely Differing Characteristics. This innovative methods recovered a higher percentage of actinomycete diversity from a subset of three soils. Recommendations include the assessment of soil characteristics prior to selecting the optimal protocol for soil DNA extraction and purification.
http://aem.asm.org/content/74/9/2902.full
I am providing a link for a paper on Innovative Methods for Soil DNA Purification Tested in Soils with Widely Differing Characteristics. This innovative methods recovered a higher percentage of actinomycete diversity from a subset of three soils. Recommendations include the assessment of soil characteristics prior to selecting the optimal protocol for soil DNA extraction and purification.
http://aem.asm.org/content/74/9/2902.full
Thank you for giving this information. However, I am looking for the method of organic matter purification (including 2 fractions: humic and fulvic acid).
The organic matter can be extracted and purify from soil using the reagents like 0.5 N NaOH and 0.1M Na4P2O7 on the basis of solubility characteristics.The SOM is sub-divided into three classes of humic material: 1. Fulvic acid, which is soluble in alkali and acids, 2. Humic acid, which is soluble in alkali but insoluble in acid and 3. Humin, which is insoluble in both alkali and acid. To purify the organic matter first soil has to reflux with methanol/benzene. The soluble portion are then non-humic substances which can be discarded. The residue portion will be Humic substances. The residue portion can then extract with alkali. You may get insoluble portion , which is nothing but Humin fraction. The soluble portion then treat with acid. Here the non-precipitate portion will be Fulvic acid fractions and precipitated part will be Humic acid fractions.
Organic matter can be fractionated using alkaline sodium pyrophosphate.(0.1 M) solution. Generally soil to extractant ratio is 1:5. Shake the contents for 2-3 h on shaker for the extraction to be complete. Let the contents settle for 24 h and filter through pre-weighed Whatman filter paper 42. Residue will be humin. Acidify the filtrate with H2SO4 to pH 2.0. Fulvic acid will be in the solution and humic acid fractions will be precipitated.
Thank you guys for answering my question.
@Tarafdar, has the method of purifying SOM as you mentioned been recommended by any papers? If possible, can you share with me some papers that used this method?
@Sunita: I know some papers provided SOM extraction method, I am just not clear about the SOM purification method.
Dr. Dam it is the concept and standard methods for different organic matter fractions. You may find it any soil analysis book.
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