Hello,

I am currently exploring options for performing gene knockout in Clostridium species and would appreciate your expertise on the most suitable method for this task. Specifically, I am aware of these following approaches:

  • ClosTron (Group II Intron-Based System)
  • Small RNA-Based Systems
  • CRISPR-Cas9
  • Type V CRISPR CAS
  • Which will be best for efficient knock out to sudy clostridium specis.

    Thanks,

    Poushali Bose

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