Does anyone have a protocol to transient transfect HEK293 with 2 plasmids?

I need to transient transfect HEK293 cells with a plasmid containing a luciferase tag and a plamid containing a histamine receptor. I have never done this before so I am looking for a straight...

08 September 2020 845 3 View

How to apply thermal and structural loading for each time step in thermal-structural analysis in ANSYS APDL?

I'm simulating an experiment in which a wood beam is submitted to thermal and structural loads at the same time. The beam is put in a furnace where tempeature rises according to ISO curve and at...

18 June 2018 8,802 9 View

Does anyone know if pKD46 system works in E. coli BL21 or DH5alpha to KO a gene?

I need to KO a gene in my bacteria and I'm currently use BL21 strain also for the over expressed one. I've been trying many systems to ko my gene but so far I didn't get any good result and I'm...

03 June 2018 2,089 6 View

Protocol PCR primers narG1950m & narG2050m?

Did someone used these primers in PCR or qPCR? Because I have a problem to perfom the PCR and I obtained the no appropriate band in the samples and NTC. Thanks Giorgia

29 January 2018 1,404 3 View

Non-neurotoxic brain dye for infusion?

Dear all, I'm looking for a non neurotoxic brain dye that I can infuse into the ventricles through osmotic pumps, to be sure that I'm doing the infusion in right way. The mice must survive for at...

29 August 2017 5,509 3 View

Renal sympathetic nerve staining

I know it's commonly done with tyrosine hydroxilase but I was looking for an alternative IHC (less expensive) method. I came to understand silver staining or Luxol staining are possibilities but I...

12 June 2017 6,112 2 View

It is possible to express the increase of the expression of a microRNA as the sum of its intracellular content and the one in the supernatant?

I need to quantify the total expression of a miRNA that is contained in a cell and is also released from it in the culture medium. How can I do? Do You know a few articles in the literature that...

12 December 2016 1,073 3 View

RT-qPCR protocol one or two steps?

Is best to prepare the RT-qPCR in one-step or two-step?

26 November 2016 9,555 8 View

I'm looking for an online atlas ofthe human brain that allows input of xyz coordinates in MNI space to determine and label corresponding brain areas.

Can anyone point me in the right direction here please? many thanks

11 September 2013 6,106 11 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Genome-wide gene identification protocol ?

Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?

28 February 2021 8,095 1 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

How to add missing citation?

Dear colleagues, I have found that many authors have a concern regarding their missing citation in RG because often citation in RG is lower than google scholars. I also missed some citation...

26 February 2021 928 6 View

How to compare 2 samples recruited with different sampling methods?

If I want to compare characteristics of 2 groups of patients, one group is recruited from a random sample of the general public while another group is from a sample with a fixed gender (male to...

26 February 2021 3,526 3 View

What is the most suitable medium of ascomycetes fungi ??

What is the most suitable medium of ascomycetes fungi ??

26 February 2021 4,413 1 View

Centrality measures computation in Biblioshiny/ Vosviewer?

26 February 2021 7,279 2 View

RNA-seq DEGs downstream analysis and reporting in literature?

I used EdgeR to get the list of DEGs (differentially expressed genes) from my liver RNA-seq experiment. I have done the gene overrepresentation and GSEA based pathway analysis. I am currently...

23 February 2021 8,969 1 View

Could you please suggests a tutorial or paper or any other materials to learn multiple gene expression in TCGA and GTEx databases?

I would like to analyze multiple gene expressions in normal and cancer tissue by using the data in the TCGA and GTEx databases. Please suggest a tutorial or paper showing step by step analysis of...

23 February 2021 9,007 3 View

Are there any databases for gene co-expression or expression pattern clustering?

I am currently working on gene clustering based on co-expression pattern in mouse brain. The problem is I do not have some solid way to test my result. Are there any suggestions for databases...

22 February 2021 4,374 3 View