Smering in Pcr Amplification ?

I am facing a problem regarding smearing in PCR amplification since two months. But, before six months the results was there using the same primers at 67.2-degree celsius annealing temperature, 5%...

23 November 2020 4,394 5 View

Dear all, I am interested in a project relating to agricultural waste management. Is there anyone who is interested to collaborate?

In this project on agricultural waste management, we will discuss the problem of agricultural waste, how it can be managed, and its legal framework. Area of study: Distt Patiala and Ludhina,...

13 July 2020 8,112 4 View

Whom should I approach for analysis and assembly of NGS reads of nanobodies in the camel phage display library?

We constructed Nb-phage display library derived from an LPS-immunized Indian desert camel and have recently subjected it to NGS using Illumina MiSeqTM platform. The raw data of NGS reads was...

24 May 2020 8,054 3 View

I am getting error saying could not open conf.txt for reading in AutoDock Vina.?

When I tried to docking windows cmd shows Error: could not open "conf.txt" for reading. I have tried to change the path for cmd e.c.t, but it was not helping. And I tried to change from program...

10 May 2020 8,821 4 View

Required open access software package for windows?

I am working in 2D materials, please suggest best open access software for windows to analysis of electronic, optical and thermal properties

13 April 2020 437 3 View

Determining encapsulation efficiency of Liposomes using Triton X-100??

I am trying to determine the encapsulation efficiency of liposomes using Triton-X 100 in order to disrupt the membrane followed by extraction of lipophilic drug using hexane..Whenever i put hexane...

30 January 2020 3,404 3 View

How to change the intracellular pH in human cell line?

Hi All, I want to study the effect of change in intracellular pH. Can you suggest, how can I change the intracellular pH in human cell line. Is there any specific cell line suitable for such...

17 July 2019 1,054 1 View

Poor area regeneration in case of HPLC in water as a solvent ?

I am working in oxidation reaction. I am facing a problem regarding product quantification. When i used water as a solvent then converted area of my reactant is not able to regenerate in product...

21 April 2019 5,742 5 View

How many iterations are generally required to get convergence for PEMFC simulations in ANSYS FLUENT ?

14 February 2019 849 4 View

Where can i find PCG(Phonocardiogram) with ECG(Electrocardiogram) database?

I found some ECG along with PCG from Physionet 2016 challenge dataset with name "a". But, description of ECG is unknown hence i am unable to apply particular algorithm to detect important...

24 December 2018 1,017 5 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

How to separate water from glycerol solvent in a solvent recovery vacuum distillation column?

I am currently doing my third year design project. I have been assigned to design a glycerol recovery column in vinyl acetate monomer production. The process unit before the recovery column is an...

01 March 2021 9,525 4 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

POlymer+DMSO solution and moisture??

What could be the reason of moisture at the sides of Vial when I am preparing my polmyer+DMSO solution for membrane fabrication? What etiquettes I am possibly missing here?

28 February 2021 6,516 5 View

Multi effect distillation modeling in EES?

Dear colleagues If anyone has a model of multi-effect distillation in EES or MATLAB software, please send it to me.

24 February 2021 7,282 1 View

IF troubleshooting: looking at an intracellular protein that localizes to the cell's and how to reduce increase in signal due to cell ruffles?

I am currently trying to stain for PI3KBeta in MDA-MB-231 cells. My current issue is that 1) beta stains all over the cell and 2) there is an increase in signal at the edge of the cell and this...

24 February 2021 8,582 1 View

Cloudy/high background western blot - can anyone help me?

Hi everyone! I try to detect VDR protein (~48 kDa) using Western Blot. After SDS-PAGE using 10% MP TGX Stain-Free Gel from BioRad I transferred the protein onto an metOH activated PVDF membrane...

23 February 2021 6,266 3 View

What is the best etching method for Mg-Ca alloy?

I have used Picral etchant (4.2 g Picric acid, 10 mL Acetic acid, 70 mL Ethanol, 10 mL distilled water) but it didn't work. Is there any alternative?

21 February 2021 2,558 5 View

Protocol for membrane extraction E. coli?

Hello! I am interested in determining whether a poly-unsaturated fatty acid I have engineered E. coli to produce is being incorporated into the membrane of the cells or if it is simply a free...

21 February 2021 7,447 2 View