I have previously used LB broth medium in biofilm inhibition experiments against P. aeruginosa. However, I could not detect an effective biofilm formation in the negative control wells. Which medium would you recommend for sufficient biofilm formation in P. aeruginosa?
Thank you.
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You may try no NaCl LB that only contains tryptone and yeast extract. Also PI medium (Pseudomonas Isolation) is also widely used in biofilm study.
Back to your experiment, if you use WT PA like PAO1 as negative control, please be noted that WT starin usually do not produce lots of biofilm.
Hope it helps you!
Fuat Tüfekci No problem. You can also extend incubation time to 48 or 96 h to get more biofilm.
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