Which markers can I use to stain microglia in paraffin sections?

More Anne Gro's questions See All

How to find the mean, SD and CI in SPSS?

Hi, I hope that someone can help me with this task: I have 5 groups of participants which are numbered in SPSS from 1 to 5 (according to their group number) and are all under the variable group....

03 March 2021 506 3 View

Can cells be reused after using WST 8/ CCK 8 from Sigma Aldrich?

Hi all, Can cells be reused after I have seed them onto 96 well plates and test them with cell counting kit at OD450 (CCK8) at 12 hours, wash them with PBS x3 and add new media and let them...

24 February 2021 5,072 1 View

How do I change a preprint to published?

I want to know how to change the status of an article.

21 February 2021 1,536 2 View

What buffer to use for biotinylation as alternative to PBS?

I'm looking to biotinylate a protein that precipitates in PBS. The recommended reconstitution buffer for the protein is urea/Tris buffer, pH 7,5 (7 M urea, 5 mM EDTA, 15 mM mercaptoethanol, 20 mM...

04 January 2021 7,681 5 View

Absolute Quantification: Serial dilution resulted in same CT values?

Hello, we are currently working on absolute quantification with SYBR Green qPCR. We excised from a plasmid containing the cDNA, a fragment, which will be amplified by the primers we used in our...

10 November 2020 769 4 View

What would cause Cell Titre Glo assay wells to luminesce less than the media only wells?

Hi all, I'm doing a bunch of Cell Titre Glo assays to assess the effects of several inhibitors on 2 cell lines. Problem is, for some of the wells they seem to be luminescing less than the...

30 October 2020 1,513 2 View

Can you recommend a reliable AMH ELISA for rat serum/plasma?

I am looking for a reliable and sensitive AMH ELISA for use with rat serum (and potentially plasma). Recommendations are welcomed! Thank you in advance.

08 July 2020 3,207 2 View

To what extend do you agree with this proposition on sustainable procurement??

" Picture a world where every product and appliance is environmentally friendly, where every supermarket item is fair trade, where corruption is an urban myth and poverty a long-distant memory....

19 May 2020 1,933 3 View

How Do You Incubate Microfluidic Chips with Cells?

Hello, I am interested in this research field. I have the prototype of my microfluidic chips, but I am having a hard time finding literature that says exactly how they incubate their chips (with...

21 April 2020 3,277 5 View

Meta-analysis on prognostic studies: How to reach an optimal cut-off value?

I'm doing a meta-analysis on prognostic studies. The studies I have included present the following values: AUC, 95% CI, a cut-off value and associated sensitivity and specificity. Also, is it...

01 March 2020 10,001 2 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View

Ammar Alhaaik

try this kit RCA-1 , Vector Laboratories,

Dhyaa Ab. Abood

Hi dear follow this topic

Maurice Shen

Hi Anne,

I'd recommend doing a search on BenchSci (https://www.benchsci.com)

Please see screenshot attached.

I hope this helps!

Maurice

Alberto Sepulveda Rodriguez

Some classic antigens with varying and contested degrees of specificity are CX3CR1, F4/80, Csf1r, CD11b/OX-42 among others. More novel ones are P2YR12 and TMEM119. There are well-cited and characterized Abs against most or all of these proteins that have been validated for IHC in paraffin embedded sections.

Alternatively, you can try to stain with tomato lectins or even the original silver carbonate method from del Rio Hortega...