Which mammalian promotor is suitable for weak but steady expression via transduction in mammalian cells?

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Cuntheepuram Lakshminarasimhan

You can try rabits by ear inoculation intravenous . It may take three weeks to rejuvinate and produce the antiserum

Nesrin Ozoren

Why do you think CMV promoter is too strong?

Ruchan Karaman

We use EF1a or EF5a promoters for such experiments in the mouse liver. Also make sure that your polyA is not sv40 or some other viral polyA. It normally works great with Ef1a promoter and Bgh polyA.