Determinations of total carotenoids and chlorophylls a and b ...
www-06.all-portland.net/bst/011/0591/0110591.pdf
by K HARTMUT
Various equations for the determinations of total chlorophyll and individual ... relative specific absorption coefficients to these values were established ... acetone, 80% (v/v) acetone, 96% (v/v) ethanol and methanol. The red ... values at 470nm for total leaf carotenoids [xanthophyll (x)plus carotenes (c)] in direrent solvents.
It's hard to find the specific absorbance coefficient for chlorophyll c in methanol at 470 nm. In Fucales or in Brown algae, different authors estimates carotenoids using chlorophyll a and b equations, but I think that it's wrong... I'll study this topic and try to propose the solution.