Hank’s balanced saline solution (HBSS) is the best medium to preserve elastic properties of hard tissues (Raum et al., 2007)

Raum K, Kempf K, Hein HJ, Schubert J and Maurer P. 2007. Preservation of microelastic properties of dentin and tooth enamel in vitro-a scanning acoustic microscopy study. Dental Materials. 23 (10), pp.1221-1228.

Thank you very much for your help Mr. Sindi. Have a nice evening.

Anytime! Have a great weekend.

Room temperature whole milk is very adequate to for the short term, but the best medium is the freshly created recipient site in the alveolar crest.

The following is an excellent series on autogenic transplants. The first and second ones do a good job on preservation - if my memory serves we well.

Andreasen JO, Paulsen HU, Yu Z, Ahlquist R, Bayer T, Schwartz O. A long-term study of 370 autotransplanted premolars. Part I. Surgical procedures and standardized techniques for monitoring healing. Eur J Orthod. 1990 Feb;12(1):3-13.

Andreasen JO, Paulsen HU, Yu Z, Bayer T, Schwartz O. A long-term study of 370 autotransplanted premolars. Part II. Tooth survival and pulp healing subsequent to transplantation. Eur J Orthod. 1990 Feb;12(1):14-24.

Andreasen JO, Paulsen HU, Yu Z, Schwartz O. A long-term study of 370 autotransplanted premolars. Part III. Periodontal healing subsequent to transplantation. Eur J Orthod. 1990 Feb;12(1):25-37.

Andreasen JO, Paulsen HU, Yu Z, Bayer T. A long-term study of 370 autotransplanted premolars. Part IV. Root development subsequent to transplantation. Eur J Orthod. 1990 Feb;12(1):38-50.

Good luck

Using Extracted Teeth for Research

The Effect of Storage Medium and Sterilization on Dentin Bond Strengths

JADA Volume 138, Issue 12, Pages 1599–1603

The Centers for Disease Control and Prevention has adopted guidelines for infection control of extracted teeth used for research and teaching, requiring that teeth be sterilized before use. The authors conducted a study to test the null hypothesis that the storage medium and sterilization method have no effect on composite-to-dentin bond strengths.

Materials and Methods

The authors collected 170 bovine incisors, cleaned them and placed them randomly into one of six storage media at 37°C for 60 days: distilled water (dH2O), 0.9 percent sodium chloride, 0.5 percent chloramine-T, 5.25 percent sodium hypochlorite (NaClO), 2 percent glutaraldehyde and 10 percent formalin. For sterilization, they autoclaved a subset of 10 specimens from every sample, while they stored another subset of 10 specimens from every sample (except for the 10 percent formalin sample) in 10 percent formalin for 14 days. The authors then embedded the specimens in epoxy and ground flat the facial surface to expose middle-depth dentin, which they polished to 600 grit. They used a dental adhesive to apply composite to the exposed dentin. The authors tested the composite-to-dentin shear bond strength 24 hours after bonding. They analyzed the data using global analysis of variance and, when appropriate, multiple post hoc tests (P = .05).

Results

Storage in NaClO resulted in significantly lower bond strength than that of the other treatment specimens. Sterilization with the autoclave negatively affected the bond strength of specimens stored initially in dH2O or 10 percent formalin, while sterilization with formalin alone had no significant effect on bond strengths.

Conclusions

Storing bovine teeth in 5.25 percent NaClO may negatively affect composite-to-dentin bond strengths. Immersion in 10 percent formalin might be the best option for storage and sterilization of bovine teeth that are to be used in dental bonding studies in vitro.

Thanks a lot.

hello:

we have used many techniques for storing teeth for orthodontics experiments

we have used 70 % alcohol 

1.      Chumak, L., Galil, K.A., Way, D.C., Johnson, L.N. and Hunter, W.S. (1989) an in vitro investigation of lingual bonding.  Am. J. Orthodontics 95, 20-28.

we have used refrigerated tap water and added thymol

1.      Greenlaw, R., Way, D.C. and Galil, K.A. (1989) an in vitro evaluation of a visible light-cured resin as an alternative to conventional resin bonding systems.  Am. J. Orthodontics 96, 214-220.

we also used 10% buffered formaline which also gave good results 

1.      Galil, K.A. and Wright G. (1979) Acid etching patterns of buccal surfaces of permanent teeth.  Paediatric Dentistry. I, 230-234.

2-1.      Galil, K.A. and Wright G. (1982) Comparative microscopical study of resins bonded to buccal enamel surfaces. J. Canad. Dent. Assn. 48, 51-55.

3-Galil, K.A. and Wright, G. (1979) Effects of various acids on the buccal surface of human permanent teeth. A   (S.E.M.) study. Paediatric Dentistry. I, 155-159.

4.      Galil, K.A. (1975) Histochemistry and scanning electron microscopy of tooth integumental material. J. periodont. 46, 415-420.

5      Galil, K.A. (1975) An in vitro enamel cleaning method for examination of teeth by scanning electron microscopy. J. Canad. Dent. Assn. 41, 559-562.

6.      Galil, K.A. (1975) A scanning and transmission electron microscopic study of the occlusal surface of plaque

my recommendation 

is 1- if you want use refrigerated water  use with it thymol crystals to inhibit growth of fungi but you have tio change water daily

2- if you want to use alcohol 70% that works fine'

3- if you want to use 10% buffered formalin that is fine 

all will give good results for orthodontic research based on our(my) experience

 cheers

professor Galil

Very constructive suggestions. Thanks All

https://www.sciencedirect.com/science/article/abs/pii/S0002817714638071

Storage of extracted teeth:

a)Upon extraction: Store teeth in 0.5% Chloramine-T immediately after extraction.

b) Upon receipt in the laboratory: Remove adherent tissue, clean teeth and place in fresh 0.5% Chloramine-T and store at 4–7 °C.

c) Tooth hydration: Do not allow the tooth to dehydrate from point of extraction throughout the entire experimental process (continuously immerse in aqueous media without antimicrobial agent or cover with wet tissue paper in between the processing steps).

d) Aqueous storage media type: Laboratory grade water is not necessary, but if using deionized water be aware that tooth surface demineralization has been documented, therefore a phosphate-buffered salt solution of neutral pH, e.g. Dulbecco’s Phosphate-Buffered Saline (DPBS), Hank’s balanced salts solution (HBSS) is recommended.

From: Article Academy of Dental Materials guidance on in vitro testing of ...

Dear Cihan Aydoğan thank you so much for bringing in this topic of storing the extracted teeth. We usually place them in a 10% formalin solution, but this discussion has given me valuable ideas.

thanks and regards

Amina Sultan

CloT may interfere with bonding tests. I do not like it!!

Tore, but that is the recommended protocol of the ADM: Academy of Dental Materials guidance on in vitro testing of dental composite bonding effectiveness to dentin/enamel using micro-tensile bond strength (µTBS) approach. What other methods would you suggest?

hello: we have used many techniques for storing teeth for orthodontics experiments we have used 70 % alcohol  1.      Chumak, L., Galil, K.A., Way, D.C., Johnson, L.N. and Hunter, W.S. (1989) an in vitro investigation of lingual bonding.  Am. J. Orthodontics 95, 20-28. we have used refrigerated tap water and added thymol 1.      Greenlaw, R., Way, D.C. and Galil, K.A. (1989) an in vitro evaluation of a visible light-cured resin as an alternative to conventional resin bonding systems.  Am. J. Orthodontics 96, 214-220. we also used 10% buffered formaline which also gave good results  1.      Galil, K.A. and Wright G. (1979) Acid etching patterns of buccal surfaces of permanent teeth.  Paediatric Dentistry. I, 230-234. 2-1.      Galil, K.A. and Wright G. (1982) Comparative microscopical study of resins bonded to buccal enamel surfaces. J. Canad. Dent. Assn. 48, 51-55. 3-Galil, K.A. and Wright, G. (1979) Effects of various acids on the buccal surface of human permanent teeth. A   (S.E.M.) study. Paediatric Dentistry. I, 155-159. 4.      Galil, K.A. (1975) Histochemistry and scanning electron microscopy of tooth integumental material. J. periodont. 46, 415-420. 5      Galil, K.A. (1975) An in vitro enamel cleaning method for examination of teeth by scanning electron microscopy. J. Canad. Dent. Assn. 41, 559-562. 6.      Galil, K.A. (1975) A scanning and transmission electron microscopic study of the occlusal surface of plaque my recommendation  is 1- if you want use refrigerated water  use with it thymol crystals to inhibit growth of fungi but you have tio change water daily 2- if you want to use alcohol 70% that works fine' 3- if you want to use 10% buffered formalin that is fine  all will give good results for orthodontic research based on our(my) experience  cheers

and finally if you do not like to have any soft tissue attached to the tooth before testing for bonding or deponding or for scanning electron microscopy we have published a paper to show that 5% sodium hypochlorite will do the job efficiently but please look at our paper which we have included here so that you can see the effect o sodium hypochlorite .outr SEM were excellent with no traces of any soft tissue left over.

professor Galil

Dr.K.A.Galil.Professor of Medicine &Dentistry DDS.,D.Oral & Maxillofacial Surgery,PH.D,FAGD.,FADI.,Cert.Periodontist(Royal College of

dental surgeons )

http://www.drgalil.ca/index.php http://www.drgalil.ca/fifty_one.php