AOAC Official Method 995.03; Ac cu rately weigh ca 25 g pep per
into 500 mL boil ing flask. Add 200 mL eth a nol and sev eral glass beads, and at tach flask to re flux con denser. Gently re flux 5 h and let cool. Fil ter 1–4 mL solution through 0.45 mm sy ringe fil ter into small glass vial.
Search articles about capsaicin in Google Scholar, there are so many methods to extract it, it seems that Supercritical Fluid Extraction is better.
In our research, we found ethyl acetate a very effective solvent to extract capsaicin from ground dried green chilli, Capsicum annuum.
Dear James Villar,
Methanol is not a good choice, Dichloromethane or Ethyl Acetate is best. Preferably, I agreed with Dildar Ahmed with some modification. After adding Ethyl Acetate please perform Sonication for 4 hours at 25 Celsius.
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