Intuitively, I would assume ethanol. If you go back to the fundamentals, the longer hydrophobic ethyl should furnish a wider gamut of potential binding partners. If you think about it conceptually, good detergents have a very hydrophilic head and a long hydrophobic tail. The former will readily form hydrogen bonds with water while the latter exhibits strong intermolecular attraction to the hydrophobic oils.
Having a farther "reach" with an ethyl moiety would, in this researchers opinion, garner a greater quantity of potential binding partners than methanol (greater distance, less electrostatic repulsion, and the other associated phenomena), the latter of which exhibits far more pronounced hydrophilic properties; and is thus more akin to the action of water than other alcohol organic solvents.
Dear Muhamad, which is the nature of your samples? Biofluids (plasma, urine..), tissue, cells? Different matrices need different extraction procedures. Moreover, you want polar or non-polar metabolites? or both?
Hi Claudia Fattuoni , thanks for joining the discussion room. It is a mushroom tissue. I need to extract both polar and non-polar metabolites. Kindly elaborate more on the extraction procedure and the suitable solvent to extract the chemicals. Thank you.