You can use R program .

The MRtrix3 software package includes a suite of tools for image analysis and visualisation. With the exception of mrview and shview, all MRtrix3 executables are designed to be run via a terminal using a consistent commandline interface. While many of the tools and features are discussed within tutorials found in this documentation, a comprehensive List of MRtrix3 commands can be found in the reference section. These lists provide links to the help page (manual) for each executable, which can also be accessed by typing the -help option after the executable name on the terminal.

https://readthedocs.org/projects/mrtrix/downloads/pdf/latest/

_____

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3709076/

_____

Statistical Analysis

Given the wide age range in our study population and the previously documented age-related changes in diffusion parameters in white matter (19), we corrected parameter values in the NAWM and PLIC for age, on the basis of the average age of the complete patient group (54 years) and the slope of the linear regression of the respective parameter value versus age in the NAWM or PLIC. Statistical analysis was performed by three authors (S.V.C., R.R.P., and F.D.K. [with 10 years of experience]).

MD, FA, radial kurtosis, axial kurtosis, and mean kurtosis and the corresponding normalized values to the contralateral NAWM in solid tumoral tissue and values in edema, NAWM, NAGM, and PLIC were compared among the high-grade gliomas and the low-grade gliomas by using the Mann-Whitney-Wilcoxon test with Bonferroni correction. The statistical threshold for significance was P