Bacterial small RNA sequencing
hello everyone, Anyone can help me fix that error that occurred when I was running PGLS model in caper package .
21 February 2024 3,475 0 View
Does it matter how GPS coordinates are reported in field biology - as in, is there a general preference between reporting GPS data using sexagesimal degrees (Degrees, Minutes, Seconds) or decimal...
12 January 2024 9,045 2 View
One of the factors that can affect the quality control of the laboratory is the laboratory workers. However, some laboratory workers do not follow the procedures for various reasons. What methods...
03 September 2023 3,548 3 View
I am considering doing some research on the etymological origins of certain words related to student government and student leadership, particularly words such as "student", "pupil", etc., to...
08 August 2023 4,539 6 View
I am currently trying to just prime my Biotage Isolera but it keeps giving me an error saying pump A is stuck. I tried cleaning the check valve to see if that will clean anything, it did not....
22 June 2023 569 2 View
Do academic hiring committees consider book reviews? I enjoy doing book reviews because I get free books, small publications, and it allows me to stay up to date on recent scholarship in my...
21 May 2023 7,997 9 View
Does anyone know how, after importing a correlation matrix in R, I can specify the # of observations?
22 February 2023 9,972 1 View
What was your experience searching for academic jobs for the first time? Were you able to obtain a tenure-track position? How many jobs did you apply to, where did you apply, and what was the...
14 January 2023 4,928 5 View
A geophysical model is obtained before inversion process.
11 December 2022 4,645 3 View
The above question is part of the workflow for inversion process using 3D seismic wave data
11 December 2022 8,394 2 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...
10 August 2024 8,539 5 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...
06 August 2024 807 3 View
I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...
06 August 2024 1,367 3 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
What is the most suitable experimental setting to understand the consequences of a particular gene: (1) targeted degradation of the specific transcription factor (TF) of the gene, followed by...
04 August 2024 6,265 1 View
