Bacterial small RNA sequencing
Hello all, I am interested in deleting a highly expressed non-coding RNA located on a Listeria monocytogenes plasmid. Currently, there are not any optimized gene deletion methods for Listeria...
09 February 2021 4,994 1 View
Can anyone provide any insight as to why my Tris/Gly SDS-Gels are polymerizing slowly? This especially applies to my stacking gel (image attached) both 4% and 6%. The stacking gel wells polymerize...
24 January 2021 3,791 4 View
we plan to induce colon cancer by injection. Cell lines derive from BDIX rats, but our experimental setting would favour SD rats. any experience?
23 January 2021 1,437 2 View
Assays for bacterial small RNA sequencing
06 January 2021 4,549 2 View
Dear all, Good day! Recently i tried to ferment my blended soya bean powder mixed with Natto bacteria culture in a Natto maker (containing some water) at around 45 degree celsius overnight....
23 November 2020 7,105 20 View
I am looking to complete western blot experiments and IHC experiments on the same cohort of mice. Is it possible to Harvest the mouse and use the right hemisphere for IHC and the left for western...
08 September 2020 5,436 3 View
Dear all, Recently i have been trying to measure colour difference between my samples. I am a little confused as to why i cannot just use the basic colours - i.e., red, green and blue to measure...
02 August 2020 7,375 2 View
Hi, I am studying the knockdown of Trf2 protein in Murine microglia cells invitro and wish to demonstrate this knockdown via immunofluorescence and Western blot. A dependable Trf2 antibody and...
02 June 2020 8,682 3 View
Hi, I'm trying to merge two chlorophyll datasets from different sensors by applying linear regression on their data overlap. The journal that I'm following log transformed (base 10) the data from...
02 April 2020 871 7 View
Hello, I am new to sequencing and this line of work. For the past several weeks I have been using Geneious, and have collected several sequences that I believe to be a certain protein group. I...
29 March 2020 1,189 3 View
I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance
03 March 2021 3,568 1 View
Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue
02 March 2021 5,433 5 View
I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....
01 March 2021 3,607 3 View
I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...
28 February 2021 4,949 3 View
I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...
27 February 2021 356 6 View
Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...
27 February 2021 9,233 3 View
I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...
27 February 2021 547 3 View
Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...
25 February 2021 6,969 3 View
I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...
24 February 2021 4,397 3 View
I have synthesised cDNA from 500ng/ul of RNA. The concentration of the cDNA Is about 5000ng/ul. Should I dilute my cDNA or just use it as it is for my qPCR? I usually use 2ul of cDNA for a 20ul...
24 February 2021 8,618 3 View
