I'm actually working in human macrophages HCMV infection. I'm used to calculate percentage of infection by fluorescence microscopy, using indirect staining and counting IE1+ cells. Is this method proper to know whether cells are more or less permissive to infection in any conditions? Do you use other method to calculate it, instead? I only want to be sure enough to stablish than lower IE1+ cells means lower infection. Thank you very much in advance!
In my opinion IE1 expression in the cell of interest is the best way to see the number of cells infected since it is a direct indication of active viral replication. If you have titrated the viral prep in different cell type, its good to derive a correlation between the MOI to the target cell type, in your case the macrophages. Usually the viral preps have considerable amount of dense bodies, try and make a bigger batch of good viral prep that can be used for the whole set of experiments.
BENVOLGUT Jordi Pou,
Des de el punt de vista clínic, es útil poder estudiar la carga viral i també els anticossos IgM anti-CMV.
Varios laboratoris a Barcelona poden fer aquestes determinacions.
Cordialment,
M. Rosa Fenoll Brunet
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